Rabbit MYOG Polyclonal Antibody | anti-MYOG antibody

MYOG Antibody (Center)

Cat. Num. AAA288666

• Gene Names: MYOG; MYF4; myf-4; bHLHc3
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: MYOG, Antibody; MYOG Antibody (Center); Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4; MYOG; BHLHC3; MYF4; anti-MYOG antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MYOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-150 amino acids from the Central region of human MYOG.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 123-150
• Sequence Length: 224

• Applicable Applications for anti-MYOG antibody: ELISA, WB (Western Blot)
• Function: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth ; phosphorylation by CAMK2G inhibits its transcriptional activity in response to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation . During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYODl. Together with MYF5 and MYODI, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRGI-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (EI) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).
• Cellular location: Nucleus. Note=Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRGl/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(MYOG Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MYOG antibody detected the MYOG protein (arrow).)

Related Product Information for anti-MYOG antibody

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture.It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq].

Product Categories/Family for anti-MYOG antibody

Signal Transduction

References

Gao, X., et al. J. Cell. Biochem. 110(1):162-170(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Ramamoorthy, S., et al. Am. J. Physiol. Endocrinol. Metab. 297 (2), E392-E401 (2009) :
Nanni, P., et al. Mol. Cancer Ther. 8(4):754-761(2009)
Gong, C., et al. Genes Dev. 23(1):54-66(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 18765727
• NCBI GeneID: 4656
• NCBI Accession #: NP_002470.2
• NCBI GenBank Nucleotide #: NM_002479.5
• UniProt Accession #: P15173; Q53XW6
• Molecular Weight: 25037
• NCBI Official Full Name: myogenin
• NCBI Official Synonym Full Names: myogenin (myogenic factor 4)
• NCBI Official Symbol: MYOG
• NCBI Official Synonym Symbols: MYF4; myf-4; bHLHc3
• NCBI Protein Information: myogenin
• UniProt Protein Name: Myogenin
• UniProt Gene Name: MYOG
• UniProt Synonym Gene Names: BHLHC3; MYF4; bHLHc3; Myf-4
• UniProt Entry Name: MYOG_HUMAN