WB (Western Blot)
(All lanes : Anti-MYPT1 (Ser507) Antibody at 1:4000 dilutionLane 1: 293 whole cell lysateLane 2: Hela whole cell lysateLane 3: HT-29 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: C2C12 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 115 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit MYPT1 Polyclonal Antibody | anti-MYPT1 antibody
MYPT1 (Ser507) Antibody
Gene Names
PPP1R12A; MBS; M130; MYPT1
Reactivity
Human, MousePredicted: Mouse
Applications
ELISA, Western Blot
Synonyms
MYPT1, Antibody; MYPT1 (Ser507) Antibody; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A; MBS; MYPT1; anti-MYPT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Mouse
Predicted: Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This MYPT1 (Ser507) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 481-514 amino acids from the human MYPT1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
1030
Applicable Applications for anti-MYPT1 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthesized Peptide
Antigen Source
Human
Cellular Location
Cytoplasm. Note=Along actomyosin filaments and stress fibers
Tissue Location
Expressed in striated muscles, specifically in type 2a fibers (at protein level).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-MYPT1 (Ser507) Antibody at 1:4000 dilutionLane 1: 293 whole cell lysateLane 2: Hela whole cell lysateLane 3: HT-29 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: C2C12 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 115 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-MYPT1 (Ser507) Antibody at 1:4000 dilutionLane 1: 293 whole cell lysateLane 2: Hela whole cell lysateLane 3: HT-29 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: C2C12 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 115 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-Phospho-MYPT1 (Ser507) Antibody: Ctrl at 1:4000 dilutionLane 1: 293 whole cell lysateLane 2: Hela whole cell lysateLane 3: HT-29 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: C2C12 whole cell lysateLane 6: NIH/3T3 whole cell lysateLane 7: Mouse uterus lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 115 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-Phospho-MYPT1 (Ser507) Antibody: Ctrl at 1:4000 dilutionLane 1: 293 whole cell lysateLane 2: Hela whole cell lysateLane 3: HT-29 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: C2C12 whole cell lysateLane 6: NIH/3T3 whole cell lysateLane 7: Mouse uterus lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 115 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MYPT1 antibody
Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Product Categories/Family for anti-MYPT1 antibody
References
Takahashi N.,et al.Genomics 44:150-152 (1997). Guo J.H.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases. Xia D.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45 (2004). Scherer S.E.,et al.Nature 440:346-351 (2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
115281 Da
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 12A isoform a
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory subunit 12A
NCBI Official Symbol
PPP1R12A
NCBI Official Synonym Symbols
MBS; M130; MYPT1
NCBI Protein Information
protein phosphatase 1 regulatory subunit 12A
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 12A
UniProt Gene Name
PPP1R12A
UniProt Synonym Gene Names
MBS; MYPT1; Myosin phosphatase target subunit 1
Similar Products
Product Notes
The MYPT1 ppp1r12a (Catalog #AAA290851) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYPT1 (Ser507) Antibody reacts with Human, Mouse Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MYPT1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MYPT1 ppp1r12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYPT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.