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WB (Western Blot)
(All lanes : Anti-MYSM1 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, mouse MYSM1 Polyclonal Antibody | anti-MYSM1 antibody
MYSM1 Antibody (N-term)
Gene Names
MYSM1; 2ADUB; 2A-DUB
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MYSM1, Antibody; MYSM1 Antibody (N-term); Histone H2A deubiquitinase MYSM1; 2A-DUB; 3419-; Myb-like; SWIRM and MPN domain-containing protein 1; MYSM1; KIAA1915; anti-MYSM1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MYSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-139 amino acids from the N-terminal region of human MYSM1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
111-139
Sequence Length
828
Applicable Applications for anti-MYSM1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Clone Names
RB27661
Function
Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. Required for correct regulation of hematopoiesis and lymphocyte differentiation (PubMed:28115216, PubMed:26220525).
Cellular Location
Nucleus {ECO:0000255|PROSITE- ProRule:PRU00624, ECO:0000269|PubMed:17707232}
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-MYSM1 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-MYSM1 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
FCM/FACS (Flow Cytometry)
(MYSM1 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(MYSM1 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(MYSM1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MYSM1 antibody detected the MYSM1 protein (arrow).)
WB (Western Blot)
(MYSM1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MYSM1 antibody detected the MYSM1 protein (arrow).)
WB (Western Blot)
(MYSM1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MYSM1 antibody detected the MYSM1 protein (arrow).)
WB (Western Blot)
(MYSM1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MYSM1 antibody detected the MYSM1 protein (arrow).)
Related Product Information for anti-MYSM1 antibody
Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.
References
Dephoure, N., et al. Proc. Natl. Acad. Sci. U.S.A. 105(31):10762-10767(2008)
Zhu, P., et al. Mol. Cell 27(4):609-621(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Yoneyama, M., et al. J. Mol. Biol. 369(1):222-238(2007)
Zhu, P., et al. Mol. Cell 27(4):609-621(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Yoneyama, M., et al. J. Mol. Biol. 369(1):222-238(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95032
NCBI Official Full Name
histone H2A deubiquitinase MYSM1
NCBI Official Synonym Full Names
Myb-like, SWIRM and MPN domains 1
NCBI Official Symbol
MYSM1
NCBI Official Synonym Symbols
2ADUB; 2A-DUB
NCBI Protein Information
histone H2A deubiquitinase MYSM1
UniProt Protein Name
Histone H2A deubiquitinase MYSM1
UniProt Gene Name
MYSM1
UniProt Synonym Gene Names
KIAA1915; 2A-DUB
UniProt Entry Name
MYSM1_HUMAN
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Product Notes
The MYSM1 mysm1 (Catalog #AAA286898) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 111-139. The MYSM1 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MYSM1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MYSM1 mysm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYSM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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