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WB (Western Blot)
(Dilution: WB: 1:2000All lanes : Anti-MYT1 Antibody (C-term) at 1:2000 dilutionLane 1:SK-BR-3whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size : 55kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human MYT1 Polyclonal Antibody | anti-MYT1 antibody
MYT1 Antibody (C-term)
Gene Names
PKMYT1; MYT1; PPP1R126
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MYT1, Antibody; MYT1 Antibody (C-term); Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase; PKMYT1; MYT1; anti-MYT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This MYT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T495 of human MYT1.
Purity/Purification
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applicable Applications for anti-MYT1 antibody
WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Dilution: WB: 1:2000All lanes : Anti-MYT1 Antibody (C-term) at 1:2000 dilutionLane 1:SK-BR-3whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size : 55kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB: 1:2000All lanes : Anti-MYT1 Antibody (C-term) at 1:2000 dilutionLane 1:SK-BR-3whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size : 55kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB: 1:500)
WB (Western Blot)
(Dilution: WB: 1:500)
WB (Western Blot)
(Dilution: WB 1:500All lanes : Anti-MYT1 Antibody (C-term) at 1:500 dilutionLane1:Mouse testis lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size : 55kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB 1:500All lanes : Anti-MYT1 Antibody (C-term) at 1:500 dilutionLane1:Mouse testis lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size : 55kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB 1:500All lanes : Anti-MYT1 Antibody (C-term) at 1:500 dilutionLane 1 Mouse testis lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size : 55kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB 1:500All lanes : Anti-MYT1 Antibody (C-term) at 1:500 dilutionLane 1 Mouse testis lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size : 55kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MYT1 antibody
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Transcript Variant: This variant (1) encodes the longer isoform (1).
Product Categories/Family for anti-MYT1 antibody
References
Dai, X., et al., J. Invest. Dermatol. 122 (6):1356-1364 (2004).Nakajima, H., et al., J. Biol. Chem. 278 (28):25277-25280 (2003).Passer, B.J., et al., Proc. Natl. Acad. Sci. U.S.A. 100 (5):2284-2289 (2003).Okumura, E., et al., Nat. Cell Biol. 4 (2):111-116 (2002).Booher, R.N., et al., J. Biol. Chem. 272 (35):22300-22306 (1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,284 Da
NCBI Official Full Name
membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 3
NCBI Official Synonym Full Names
protein kinase, membrane associated tyrosine/threonine 1
NCBI Official Symbol
PKMYT1
NCBI Official Synonym Symbols
MYT1; PPP1R126
NCBI Protein Information
membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
UniProt Protein Name
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
UniProt Gene Name
PKMYT1
UniProt Synonym Gene Names
MYT1
UniProt Entry Name
PMYT1_HUMAN
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Product Notes
The MYT1 pkmyt1 (Catalog #AAA295826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYT1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYT1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the MYT1 pkmyt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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