Rabbit NACA Polyclonal Antibody | anti-NACA antibody

Phospho-NACA (Ser43) Antibody

Cat. Num. AAA329821

• Gene Names: NACA; HSD48; NACA1; skNAC
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Chicken (86%)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: NACA, Antibody; Phospho-NACA (Ser43) Antibody; Alpha NAC; Alpha-NAC; Hom s 2.02; HSD48; MGC117224; NAC-alpha; naca; NACA/1.9.2; NACA_HUMAN; NACA1; Nascent polypeptide associated complex alpha polypeptide; Nascent polypeptide associated complex alpha subunit; Nascent polypeptide associated complex subunit alpha; Nascent polypeptide-associated complex subunit alpha; anti-NACA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Chicken (86%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-NACA (Ser43) Antibody detects endogenous levels of NACA only when phosphorylated at Ser43.; Tissue Specificity: Ubiquitously expressed.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NACA antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human NACA around the phosphorylation site of Ser43.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.
• Subunit Structure: Interacts with TBP and JUN (By similarity). Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit and contacts the ribosomal protein L23, which is positioned near the exiting site. Both subunits can contact nascent polypeptide chains. NACA may also form homodimers, and only this form binds DNA.
• Similarity: The positively charged inner surface of the NAC-A/B domain is crucial for NACA localization in the nucleus and DNA-binding. This region is blocked from binding nucleic acids in the heterodimeric complex by a helix region in the beta-subunit, it also displays much higher affinity for RNA than DNA.Belongs to the NAC-alpha family.
• Subcellular Location: Cytoplasm. Nucleus.; Note: The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis NACA (Phospho-Ser43) using heatshock treated HT29 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-NACA antibody

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.

NCBI and Uniprot Product Information

• NCBI GI #: 163965364
• NCBI GeneID: 4666
• NCBI Accession #: NP_001106673.1
• NCBI GenBank Nucleotide #: NM_001113202.1
• UniProt Accession #: Q13765; Q3KQV4; Q53A18; Q53G46; A8MTN7; B2R4P8; F8VU71; E9PAV3
• Molecular Weight: 94,681 Da
• NCBI Official Full Name: nascent polypeptide-associated complex subunit alpha isoform b
• NCBI Official Synonym Full Names: nascent polypeptide-associated complex alpha subunit
• NCBI Official Symbol: NACA
• NCBI Official Synonym Symbols: HSD48; NACA1; skNAC
• NCBI Protein Information: nascent polypeptide-associated complex subunit alpha; NAC-alpha; alpha-NAC, muscle-specific form; nascent-polypeptide-associated complex alpha polypeptide
• UniProt Protein Name: Nascent polypeptide-associated complex subunit alpha
• UniProt Gene Name: NACA
• UniProt Synonym Gene Names: NAC-alpha
• UniProt Entry Name: NACA_HUMAN