WB (Western Blot)
(Detection of human and mouse NACC2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NACC2 antibody AAA213042 (lot AAA213042-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse NACC2/BTBD14A Polyclonal Antibody | anti-NACC2/BTBD14A antibody
Rabbit anti-NACC2/BTBD14A Antibody, Affinity Purified
Gene Names
NACC2; RBB; BEND9; NAC-2; BTBD14; BTBD31; BTBD14A
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NACC2/BTBD14A, Antibody; Rabbit anti-NACC2/BTBD14A Antibody, Affinity Purified; BTBD31; NAC-2; transcription repressor with a BTB domain and a BEN domain; repressor with BTB domain and BEN domain; RBB; nucleus accumbens-associated protein 2; BTBD14A; BTBD14; BTB/POZ domain-containing protein 14A; BTB (POZ) domain containing 14A; BEND9; BEN domain containing 9; NACC family member 2, BEN and BTB (POZ) domain containing; Nucleus accumbens-associated protein 2; NACC family member 2; anti-NACC2/BTBD14A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NACC2/BTBD14A antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 175 and 225
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NACC2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NACC2 antibody AAA213042 (lot AAA213042-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse NACC2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NACC2 antibody AAA213042 (lot AAA213042-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NACC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NACC2 antibody AAA213042 (lot AAA213042-1) used for IP at 6 ug per reaction. For blotting immunoprecipitated NACC2, AAA213042 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NACC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NACC2 antibody AAA213042 (lot AAA213042-1) used for IP at 6 ug per reaction. For blotting immunoprecipitated NACC2, AAA213042 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-NACC2/BTBD14A antibody
NACC2/BTBD14A functions as a transcriptional repressor through its association with the NuRD complex. NACC2/BTBD14A recruits the NuRD complex to the promoter of MDM2, leading to the repression of MDM2 transcription and subsequent stability of p53/TP53 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96BF6].
Product Categories/Family for anti-NACC2/BTBD14A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,837 Da
NCBI Official Full Name
nucleus accumbens-associated protein 2
NCBI Official Synonym Full Names
NACC family member 2, BEN and BTB (POZ) domain containing
NCBI Official Symbol
NACC2
NCBI Official Synonym Symbols
RBB; BEND9; NAC-2; BTBD14; BTBD31; BTBD14A
NCBI Protein Information
nucleus accumbens-associated protein 2; BEN domain containing 9; BTB (POZ) domain containing 14A; BTB/POZ domain-containing protein 14A; transcription repressor with a BTB domain and a BEN domain
UniProt Protein Name
Nucleus accumbens-associated protein 2
UniProt Gene Name
NACC2
UniProt Synonym Gene Names
BTBD14A; NAC2; RBB; NAC-2
UniProt Entry Name
NACC2_HUMAN
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Product Notes
The NACC2/BTBD14A nacc2 (Catalog #AAA213042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NACC2/BTBD14A Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NACC2/BTBD14A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NACC2/BTBD14A nacc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NACC2/BTBD14A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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