WB (Western Blot)
(Nanog (pAb) tested by Western blot. 20 ug of mouse Embryonic Stem Cell (ESC) extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
Rabbit anti-Human, Mouse Nanog Polyclonal Antibody | anti-Nanog antibody
Nanog antibody (pAb)
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
Nanog, Antibody; Nanog antibody (pAb); ENK; ecat4; 2410002E02Rik; anti-Nanog antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Nanog antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This antibody was raised against a peptide within the N-terminal region of mouse Nanog.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Nanog (pAb) tested by Western blot. 20 ug of mouse Embryonic Stem Cell (ESC) extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
WB (Western Blot)
(Nanog (pAb) tested by Western blot. 20 ug of mouse Embryonic Stem Cell (ESC) extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
IF (Immunofluorescence)
(Nanog antibody (pAb) tested by Immunofluorescence Mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were then permeabilized and blocked by incubating with Blocking Solution containing 5% serum/0.1% Triton X-100 in D-PBS for 2 hours at room temperature. Cells were then incubated with Nanog antibody at 1:200 dilution overnight at 4 degree C, washed with D-PBS, and incubated for 2 hours at room temperature with goat anti-mouse Cy3 secondary antibody at 1:250 dilution. Cells were visualized using a Zeiss fluorescent microscope at 20X magnification. Images show that Nanog antibody specifically stains mESC colonies and does not stain MEFs. Absence of Nanog staining in a subset of cells within the colonies suggests differentiation. Scale bars, 100 ?m.)
IF (Immunofluorescence)
(Nanog antibody (pAb) tested by Immunofluorescence Mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were then permeabilized and blocked by incubating with Blocking Solution containing 5% serum/0.1% Triton X-100 in D-PBS for 2 hours at room temperature. Cells were then incubated with Nanog antibody at 1:200 dilution overnight at 4 degree C, washed with D-PBS, and incubated for 2 hours at room temperature with goat anti-mouse Cy3 secondary antibody at 1:250 dilution. Cells were visualized using a Zeiss fluorescent microscope at 20X magnification. Images show that Nanog antibody specifically stains mESC colonies and does not stain MEFs. Absence of Nanog staining in a subset of cells within the colonies suggests differentiation. Scale bars, 100 ?m.)
ChIP (Chromatin Immunoprecipitation)
(Nanog antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from undifferentiated hESC cells and 3 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9 million sequence tags were mapped to identify Nanog binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
ChIP (Chromatin Immunoprecipitation)
(Nanog antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from undifferentiated hESC cells and 3 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9 million sequence tags were mapped to identify Nanog binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Related Product Information for anti-Nanog antibody
Short Description: Our Nanog antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples.
Background: Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Background: Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Product Categories/Family for anti-Nanog antibody
NCBI and Uniprot Product Information
Molecular Weight
45kDa
Similar Products
Product Notes
The Nanog (Catalog #AAA60024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nanog antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nanog can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nanog, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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