Natriuretic NT
(Natriuretic Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) (Cat#AAA284191)immunohistochemistry analysis informalin fixed and paraffin embedded human kidney tissue followed bY peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC)Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human Natriuretic Peptide Receptor C Polyclonal Antibody | anti-NPR3 antibody
Natriuretic Peptide Receptor C Antibody (N-term)
Gene Names
NPR3; NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Natriuretic Peptide Receptor C, Antibody; Natriuretic Peptide Receptor C Antibody (N-term); Atrial natriuretic peptide receptor 3; Atrial natriuretic peptide clearance receptor; Atrial natriuretic peptide receptor type C; ANP-C; ANPR-C; NPR-C; NPR3; ANPRC; C5orf23; NPRC; anti-NPR3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Natriuretic Peptide Receptor C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-97 amino acids from the N-terminal region of human Natriuretic Peptide Receptor C.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Length
540
Applicable Applications for anti-NPR3 antibody
ELISA, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
Cellular location
Membrane; Single-pass type I membrane protein
Antigen Region
67-97
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Natriuretic NT
(Natriuretic Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) (Cat#AAA284191)immunohistochemistry analysis informalin fixed and paraffin embedded human kidney tissue followed bY peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC)Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Natriuretic NT
(Natriuretic Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) (Cat#AAA284191)immunohistochemistry analysis informalin fixed and paraffin embedded human kidney tissue followed bY peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC)Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Fluorescent
(Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C(N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8113a Natriuretic PeptideReceptor C (N-term) primary antibody (1:200, 2h at room temperature). For secondary antibody, 488 conjugated donkeyanti-rabbit antibody (green) was used (1:1000,1h). Nuclei were counterstained with Hoechst33342 (blue) (10 ?g/ml, 5 min). Note the highly specific localization of the Natriuretic PeptideReceptor C mainly to the mainly to the nucleus.)
Fluorescent
(Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C(N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8113a Natriuretic PeptideReceptor C (N-term) primary antibody (1:200, 2h at room temperature). For secondary antibody, 488 conjugated donkeyanti-rabbit antibody (green) was used (1:1000,1h). Nuclei were counterstained with Hoechst33342 (blue) (10 ?g/ml, 5 min). Note the highly specific localization of the Natriuretic PeptideReceptor C mainly to the mainly to the nucleus.)
Application Data
(Anti-ANPC (S82) at 1:1000dilution Lane 1: Hela whole cell lysate Lane 2:HL-60 whole cell lysate Lysates/proteins at 20?g per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(Anti-ANPC (S82) at 1:1000dilution Lane 1: Hela whole cell lysate Lane 2:HL-60 whole cell lysate Lysates/proteins at 20?g per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-NPR3 antibody
ANPC is a receptor for atrial natriuretic peptide. It does not exhibit guanylate cyclase activity. There seem to be at least three ANP receptors: two with guanylate cyclase activity (ANPA and ANPB) and one (ANPC) which is probably responsible for the clearance of ANP from the circulation without a role in signal transduction.
Product Categories/Family for anti-NPR3 antibody
References
References for protein:
1.Porter, J.G., et al., Biochem. Biophys. Res. Commun. 171(2):796-803 (1990).
2.Lowe, D.G., et al., Nucleic Acids Res. 18 (11), 3412 (1990).
References for HeLa cell line:
1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695-710. [PubMed:13052828].
2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141-50. [PubMed: 9892199].
3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277-84. [PubMed: 19450234].
4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1-8. [PubMed:20143388].
1.Porter, J.G., et al., Biochem. Biophys. Res. Commun. 171(2):796-803 (1990).
2.Lowe, D.G., et al., Nucleic Acids Res. 18 (11), 3412 (1990).
References for HeLa cell line:
1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695-710. [PubMed:13052828].
2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141-50. [PubMed: 9892199].
3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277-84. [PubMed: 19450234].
4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1-8. [PubMed:20143388].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
atrial natriuretic peptide receptor 3 isoform 2
NCBI Official Synonym Full Names
natriuretic peptide receptor 3
NCBI Official Symbol
NPR3
NCBI Official Synonym Symbols
NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23
NCBI Protein Information
atrial natriuretic peptide receptor 3
UniProt Protein Name
Atrial natriuretic peptide receptor 3
UniProt Gene Name
NPR3
UniProt Synonym Gene Names
ANPRC; C5orf23; NPRC; ANP-C; ANPR-C; NPR-C
UniProt Entry Name
ANPRC_HUMAN
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Product Notes
The NPR3 npr3 (Catalog #AAA284191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Natriuretic Peptide Receptor C Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Natriuretic Peptide Receptor C can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the NPR3 npr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Natriuretic Peptide Receptor C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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