WB (Western Blot)
(Detection of human and mouse NCBP2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCBP2 antibody AAA211923 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NCBP2 was also immunoprecipitated by rabbit anti-NCBP2 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human, Mouse NCBP2 Polyclonal Antibody | anti-NCBP2 antibody
Rabbit anti-NCBP2 Antibody, Affinity Purified
Gene Names
NCBP2; CBC2; NIP1; CBP20; PIG55
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NCBP2, Antibody; Rabbit anti-NCBP2 Antibody, Affinity Purified; NCBP interacting protein 1; nuclear cap-binding protein subunit 2; nuclear cap binding protein subunit 2, 20kDa; nuclear cap binding protein subunit 2, 20kD; PIG55; NCBP-interacting protein 1; cell proliferation-inducing gene 55 protein; CBP20; CBC2; 20 kDa nuclear cap-binding protein; NIP1; NCBP 20 kDa subunit; Nuclear cap-binding protein subunit 2; nuclear cap binding protein subunit 2; anti-NCBP2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NCBP2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 106 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NCBP2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCBP2 antibody AAA211923 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NCBP2 was also immunoprecipitated by rabbit anti-NCBP2 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human and mouse NCBP2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCBP2 antibody AAA211923 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NCBP2 was also immunoprecipitated by rabbit anti-NCBP2 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human and mouse NCBP2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-NCBP2 (Cat. No. AAA211923 Lot1) used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse NCBP2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-NCBP2 (Cat. No. AAA211923 Lot1) used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1 ug/ml). Detection: DAB)
Related Product Information for anti-NCBP2 antibody
Nuclear cap binding protein subunit 2 (NCBP2) is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP2 has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay [taken from NCBI Entrez Gene (GeneID: 22916)].
Product Categories/Family for anti-NCBP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,932 Da
NCBI Official Full Name
Homo sapiens nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1, mRNA
NCBI Official Synonym Full Names
nuclear cap binding protein subunit 2, 20kDa
NCBI Official Symbol
NCBP2
NCBI Official Synonym Symbols
CBC2; NIP1; CBP20; PIG55
NCBI Protein Information
nuclear cap-binding protein subunit 2; 20 kDa nuclear cap-binding protein; NCBP 20 kDa subunit; NCBP interacting protein 1; NCBP-interacting protein 1; cell proliferation-inducing gene 55 protein; nuclear cap binding protein subunit 2, 20kD
UniProt Protein Name
Nuclear cap-binding protein subunit 2
UniProt Gene Name
NCBP2
UniProt Synonym Gene Names
CBP20; CBP20; NIP1
UniProt Entry Name
NCBP2_HUMAN
Similar Products
Product Notes
The NCBP2 ncbp2 (Catalog #AAA211923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NCBP2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCBP2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NCBP2 ncbp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCBP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.