WB (Western Blot)
(Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000.)
Rabbit NCC Polyclonal Antibody | anti-SLC12A3 antibody
NCC Antibody: ATTO 390
Gene Names
SLC12A3; NCC; TSC; NCCT
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Synonyms
NCC, Antibody; NCC Antibody: ATTO 390; Slc12a3; anti-SLC12A3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
1030
Applicable Applications for anti-SLC12A3 antibody
IF (Immunofluorescence), WB (Western Blot)
Conjugate
ATTO 390
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
WB (Western Blot)
(Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000.)
WB (Western Blot)
(Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).)
Related Product Information for anti-SLC12A3 antibody
Background Info: Detects ~160kDa
Scientific Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
Scientific Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
Product Categories/Family for anti-SLC12A3 antibody
References
1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
Molecular Weight
110,554 Da
NCBI Official Full Name
solute carrier family 12 member 3 isoform 1
NCBI Official Synonym Full Names
solute carrier family 12 (sodium/chloride transporter), member 3
NCBI Official Symbol
SLC12A3
NCBI Official Synonym Symbols
NCC; TSC; NCCT
NCBI Protein Information
solute carrier family 12 member 3; Na-Cl symporter; Na-Cl cotransporter; thiazide-sensitive Na-Cl cotransporter; thiazide-sensitive sodium-chloride cotransporter; NaCl electroneutral thiazide-sensitive cotransporter
UniProt Protein Name
Solute carrier family 12 member 3
UniProt Gene Name
Slc12a3
UniProt Synonym Gene Names
Tsc
UniProt Entry Name
S12A3_RAT
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Product Notes
The SLC12A3 slc12a3 (Catalog #AAA103575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCC Antibody: ATTO 390 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCC can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the SLC12A3 slc12a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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