WB (Western Blot)
(Western blot analysis of NCF2 in lysates of THP-1, using NCF2 Antibody(AF4643).)
Rabbit NCF2 Polyclonal Antibody | anti-NCF2 antibody
NCF2 Antibody
Gene Names
NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (92%), Sheep (100%), Rabbit (92%), Dog (100%), Chicken (92%), Xenopus (82%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
NCF2, Antibody; NCF2 Antibody; 67kDa neutrophil oxidase factor; Chronic granulomatous disease autosomal 2; FLJ93058; NADpHoxidase activator 2; NCF-2; Ncf2; NCF2_HUMAN; Neutrophil cytosol factor 2; Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); Neutrophil NADpHoxidase factor 2; NOXA2; P67 PHOX; p67-phox; p67phox; anti-NCF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (92%), Sheep (100%), Rabbit (92%), Dog (100%), Chicken (92%), Xenopus (82%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (92%), Sheep (100%), Rabbit (92%), Dog (100%), Chicken (92%), Xenopus (82%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
NCF2 Antibody detects endogenous levels of total NCF2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NCF2 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human NCF2, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Component of an NADpHoxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9).
Similarity
The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.Belongs to the NCF2/NOXA1 family.
Subcellular Location
Cytoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of NCF2 in lysates of THP-1, using NCF2 Antibody(AF4643).)
WB (Western Blot)
(Western blot analysis of NCF2 in lysates of THP-1, using NCF2 Antibody(AF4643).)
WB (Western Blot)
(Western blot analysis of extracts from MCF7 cells, using NCF2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7 cells, using NCF2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NCF2 antibody
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADpHoxidase (necessary for superoxide production).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
59,762 Da
NCBI Official Full Name
Neutrophil cytosol factor 2
NCBI Official Synonym Full Names
neutrophil cytosolic factor 2
NCBI Official Symbol
NCF2
NCBI Official Synonym Symbols
NCF-2; NOXA2; P67PHOX; P67-PHOX
NCBI Protein Information
neutrophil cytosol factor 2; NADPH oxidase activator 2; 67 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 2; chronic granulomatous disease, autosomal 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
UniProt Protein Name
Neutrophil cytosol factor 2
UniProt Gene Name
NCF2
UniProt Synonym Gene Names
NOXA2; P67PHOX; NCF-2
UniProt Entry Name
NCF2_HUMAN
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Product Notes
The NCF2 ncf2 (Catalog #AAA329513) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCF2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (92%), Sheep (100%), Rabbit (92%), Dog (100%), Chicken (92%), Xenopus (82%) and may cross-react with other species as described in the data sheet. AAA Biotech's NCF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the NCF2 ncf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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