WB (Western Blot)
(Detection of human and mouse NCKAP5L by western blot. Samples: Whole cell lysate (10 ug) from K-562, Hep-G2, LNCaP, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCKAP5L antibody (AAA213478 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse NCKAP5L Polyclonal Antibody | anti-NCKAP5L antibody
Rabbit anti-NCKAP5L Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NCKAP5L, Antibody; Rabbit anti-NCKAP5L Antibody, Affinity Purified; centrosomal protein of 169 kDa; nck-associated protein 5-like; NCKAP5-like; KIAA1602; Cep169; FP1193; Nck-associated protein 5-like; NCK associated protein 5 like; anti-NCKAP5L antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NCKAP5L antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1025 and 1075
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NCKAP5L by western blot. Samples: Whole cell lysate (10 ug) from K-562, Hep-G2, LNCaP, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCKAP5L antibody (AAA213478 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse NCKAP5L by western blot. Samples: Whole cell lysate (10 ug) from K-562, Hep-G2, LNCaP, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCKAP5L antibody (AAA213478 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NCKAP5L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP5L antibody (AAA213478 lot 1) used for IP at 1 ug per reaction. NCKAP5L was also immunoprecipitated by rabbit anti-NCKAP5L antibody BL24502. For blotting immunoprecipitated NCKAP5L, AAA213478 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human NCKAP5L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP5L antibody (AAA213478 lot 1) used for IP at 1 ug per reaction. NCKAP5L was also immunoprecipitated by rabbit anti-NCKAP5L antibody BL24502. For blotting immunoprecipitated NCKAP5L, AAA213478 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-NCKAP5L antibody
NCKAP5L regulates microtubule organization and stabilization. Promotes microtubule growth and bundling formation and stabilizes microtubules by increasing intense acetylation of microtubules. Both tubulin-binding and homodimer formation are required for NCKAP5L-mediated microtubule bundle formation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HCH0].
Product Categories/Family for anti-NCKAP5L antibody
NCBI and Uniprot Product Information
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Product Notes
The NCKAP5L nckap5l (Catalog #AAA213478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NCKAP5L Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCKAP5L can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NCKAP5L nckap5l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCKAP5L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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