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WB (Western Blot)
(Western blot analysis of Phospho-NCoA2 (Ser698/699) in lysates of HeLa TSA 400nM 24H, using Phospho-NCoA2 (Ser698/699) Antibody.)
Rabbit NCoA2 Polyclonal Antibody | anti-NCoA2 antibody
Phospho-NCoA2 (Ser698/699) Antibody
Gene Names
NCOA2; SRC2; TIF2; GRIP1; KAT13C; NCoA-2; bHLHe75
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
NCoA2, Antibody; Phospho-NCoA2 (Ser698/699) Antibody; bHLHe75; Class E basic helix-loop-helix protein 75; glucocorticoid receptor interacting protein 1; GRIP 1; GRIP1; hTIF2; MED1; MGC138808; NCoA 2; NCoA-2; Ncoa2; NCOA2_HUMAN; Nuclear receptor coactivator 2; Oncogene FGR; p160; SRC 2; src2; Steroid receptor coactivator 2; TIF 2; TIF2; Transcriptional intermediary factor 2; anti-NCoA2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-NCoA2 (Ser698/699) Antibody detects endogenous levels of NCoA2 only when phosphorylated at Ser698/699.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1464
Applicable Applications for anti-NCoA2 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human NCoA2 around the phosphorylation site of Ser698/699.
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-NCoA2 (Ser698/699) in lysates of HeLa TSA 400nM 24H, using Phospho-NCoA2 (Ser698/699) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-NCoA2 (Ser698/699) in lysates of HeLa TSA 400nM 24H, using Phospho-NCoA2 (Ser698/699) Antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemisry)
(At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-NCoA2 antibody
Description: Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).
Subunit Structure: Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with ESR1, HIF1A, NCOA1, APEX, NR3C1, NR3C2, CARM1, RARA, and RXRA. Present in a complex containing CARM1 and EP300/P300. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with PSMB9 and DDX5. Interacts (via LXXLL 1, 2 and 3 motifs) with RORA and RORC (via AF-2 motif). Interacts with RWDD3. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Interacts with NR4A3; potentiates the activity of the NR4A3 (By similarity). Interacts with NR1H3 (PubMed:19481530).
Similarity: Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently. Belongs to the SRC/p160 nuclear receptor coactivator family.
Subunit Structure: Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with ESR1, HIF1A, NCOA1, APEX, NR3C1, NR3C2, CARM1, RARA, and RXRA. Present in a complex containing CARM1 and EP300/P300. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with PSMB9 and DDX5. Interacts (via LXXLL 1, 2 and 3 motifs) with RORA and RORC (via AF-2 motif). Interacts with RWDD3. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Interacts with NR4A3; potentiates the activity of the NR4A3 (By similarity). Interacts with NR1H3 (PubMed:19481530).
Similarity: Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently. Belongs to the SRC/p160 nuclear receptor coactivator family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 175 kDa
Predicted: 160 kDa
Predicted: 160 kDa
NCBI Official Full Name
nuclear receptor coactivator 2 isoform a
NCBI Official Synonym Full Names
nuclear receptor coactivator 2
NCBI Official Symbol
NCOA2
NCBI Official Synonym Symbols
SRC2; TIF2; GRIP1; KAT13C; NCoA-2; bHLHe75
NCBI Protein Information
nuclear receptor coactivator 2
UniProt Protein Name
Nuclear receptor coactivator 2
UniProt Gene Name
NCOA2
UniProt Synonym Gene Names
BHLHE75; TIF2; NCoA-2; bHLHe75; hTIF2
UniProt Entry Name
NCOA2_HUMAN
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Product Notes
The NCoA2 ncoa2 (Catalog #AAA328519) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-NCoA2 (Ser698/699) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's NCoA2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NCoA2 ncoa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCoA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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