IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with (MOUSE) Ncor1 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Ncor1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Ncor1 immunoreactivity is localized to nucleus significantly.)
Rabbit anti-Human, mouse NCOR1 Polyclonal Antibody | anti-NCOR1 antibody
NCOR1 Antibody (N-term)
Gene Names
NCOR1; N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109
Reactivity
Human, mouse
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NCOR1, Antibody; NCOR1 Antibody (N-term); Nuclear receptor corepressor 1; N-CoR; N-CoR1; NCOR1; KIAA1047; anti-NCOR1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NCOR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-47 amino acids from the N-terminal region of human NCOR1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
16-47
Sequence Length
914
Applicable Applications for anti-NCOR1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with (MOUSE) Ncor1 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Ncor1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Ncor1 immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with (MOUSE) Ncor1 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Ncor1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Ncor1 immunoreactivity is localized to nucleus significantly.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(The anti-NCOR1 Pab is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.)
WB (Western Blot)
(The anti-NCOR1 Pab is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.)
Related Product Information for anti-NCOR1 antibody
NCOR1 mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. The gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.
Product Categories/Family for anti-NCOR1 antibody
References
Tai, H.H., et al., Biochem. Biophys. Res. Commun. 308(1):170-176 (2003).
Ishizuka, T., et al., Mol. Cell. Biol. 23(15):5122-5131 (2003).
Loinder, K., et al., J. Steroid Biochem. Mol. Biol. 84(1):15-21 (2003).
Wang, S., et al., Oncogene 21(55):8388-8396 (2002).
Baek, S.H., et al., Cell 110(1):55-67 (2002).
Ishizuka, T., et al., Mol. Cell. Biol. 23(15):5122-5131 (2003).
Loinder, K., et al., J. Steroid Biochem. Mol. Biol. 84(1):15-21 (2003).
Wang, S., et al., Oncogene 21(55):8388-8396 (2002).
Baek, S.H., et al., Cell 110(1):55-67 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
270210
NCBI Official Full Name
nuclear receptor corepressor 1 isoform 2
NCBI Official Synonym Full Names
nuclear receptor corepressor 1
NCBI Official Symbol
NCOR1
NCBI Official Synonym Symbols
N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109
NCBI Protein Information
nuclear receptor corepressor 1
UniProt Protein Name
Nuclear receptor corepressor 1
UniProt Gene Name
NCOR1
UniProt Synonym Gene Names
KIAA1047; N-CoR; N-CoR1
UniProt Entry Name
NCOR1_HUMAN
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Product Notes
The NCOR1 ncor1 (Catalog #AAA284828) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 16-47. The NCOR1 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCOR1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NCOR1 ncor1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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