Rabbit NCR3LG1 / B7H6 Polyclonal Antibody | anti-NCR3LG1 / B7H6 antibody

Anti-NCR3LG1 / B7H6 Antibody (aa389-415) IHC-plus

Cat. Num. AAA162164

• Gene Names: NCR3LG1; B7H6; B7-H6; DKFZp686O24166
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A purified
• Synonyms: NCR3LG1 / B7H6, Antibody; Anti-NCR3LG1 / B7H6 Antibody (aa389-415) IHC-plus; Rabbit Polyclonal to Human NCR3LG1 / B7H6; Human NCR3LG1 / B7H6; anti-NCR3LG1 / B7H6 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Human NCR3LG1
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide.
• Sequence Length: 454

• Applicable Applications for anti-NCR3LG1 / B7H6 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen Species: NCR3LG1 / B7H6 antibody was raised against Human
• Epitope: aa389-415
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4°C; Long term aliquot and store at -20°C; Avoid freeze thaw cycles.

IHC (Immunohistochemisry)

(Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohiostchemistry)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(Human Parathyroid: Formalin-Fixed, Paraffin-Embedded (FFPE))

NCBI and Uniprot Product Information

• NCBI GI #: 320461732
• NCBI GeneID: 374383
• NCBI Accession #: NP_001189368.1
• NCBI GenBank Nucleotide #: NM_001202439.2
• NCBI Official Full Name: natural cytotoxicity triggering receptor 3 ligand 1
• NCBI Official Synonym Full Names: natural killer cell cytotoxicity receptor 3 ligand 1
• NCBI Official Symbol: NCR3LG1
• NCBI Official Synonym Symbols: B7H6; B7-H6; DKFZp686O24166
• NCBI Protein Information: natural cytotoxicity triggering receptor 3 ligand 1
• UniProt Protein Name: Natural cytotoxicity triggering receptor 3 ligand 1
• UniProt Gene Name: NCR3LG1
• UniProt Synonym Gene Names: B7H6; B7-H6
• UniProt Entry Name: NR3L1_HUMAN