WB (Western Blot)
(Detection of human and mouse NDUFB6 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NDUFB6 antibody AAA213264 (lot AAA213264-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse NDUFB6 Polyclonal Antibody | anti-NDUFB6 antibody
Rabbit anti-NDUFB6 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NDUFB6, Antibody; Rabbit anti-NDUFB6 Antibody, Affinity Purified; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa; B17; NADH-ubiquinone oxidoreductase beta subunit, 6; NADH-ubiquinone oxidoreductase B17 subunit; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; complex I, mitochondrial respiratory chain, B17 subunit; CI; CI-B17; complex I-B17; NADH:ubiquinone oxidoreductase subunit B6; anti-NDUFB6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NDUFB6 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine, Orangutan, Gorilla, Chimpanzee
Immunogen
Between 78 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NDUFB6 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NDUFB6 antibody AAA213264 (lot AAA213264-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse NDUFB6 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NDUFB6 antibody AAA213264 (lot AAA213264-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NDUFB6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFB6 antibody AAA213264 (lot AAA213264-1) used for IP at 6 ug per reaction. NDUFB6 was also immunoprecipitated by rabbit anti-NDUFB6 antibody For blotting immunoprecipitated NDUFB6, AAA213264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human NDUFB6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFB6 antibody AAA213264 (lot AAA213264-1) used for IP at 6 ug per reaction. NDUFB6 was also immunoprecipitated by rabbit anti-NDUFB6 antibody For blotting immunoprecipitated NDUFB6, AAA213264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-NDUFB6 antibody
NDUFB6 is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. NDUFB6 has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone [taken from NCBI Entrez Gene (Gene ID: 4712)].
Product Categories/Family for anti-NDUFB6 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NDUFB6 ndufb6 (Catalog #AAA213264) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NDUFB6 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFB6 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NDUFB6 ndufb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFB6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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