IHC (Immunohiostchemistry)
(NDUFS2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit NDUFS2 Polyclonal Antibody | anti-NDUFS2 antibody
NDUFS2 Antibody (Center)
Gene Names
NDUFS2; CI-49
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NDUFS2, Antibody; NDUFS2 Antibody (Center); NADH dehydrogenase [ubiquinone] iron-sulfur protein 2; mitochondrial; Complex I-49kD; CI-49kD; NADH-ubiquinone oxidoreductase 49 kDa subunit; NDUFS2; anti-NDUFS2 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NDUFS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human NDUFS2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
286-315
Sequence Length
457
Applicable Applications for anti-NDUFS2 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(NDUFS2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(NDUFS2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from A431, Hela cell line, mouse brain and rat brain tissue lysate(from left to right), using NDUFS2 Antibody (Center). AAA288866 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A431, Hela cell line, mouse brain and rat brain tissue lysate(from left to right), using NDUFS2 Antibody (Center). AAA288866 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-NDUFS2 antibody
NDUFS2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria.
Product Categories/Family for anti-NDUFS2 antibody
References
Saada, A., et al. Am. J. Hum. Genet. 84(6):718-727(2009) Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008) Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008) Ban, M., et al. PLoS ONE 3 (8), E2891 (2008) Triepels, R.H., et al. J. Biol. Chem. 276(12):8892-8897(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52546
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
NCBI Official Symbol
NDUFS2
NCBI Official Synonym Symbols
CI-49
NCBI Protein Information
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
UniProt Protein Name
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
UniProt Gene Name
NDUFS2
UniProt Synonym Gene Names
CI-49kD
UniProt Entry Name
NDUS2_HUMAN
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Product Notes
The NDUFS2 ndufs2 (Catalog #AAA288866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 286-315. The NDUFS2 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFS2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the NDUFS2 ndufs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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