IF (Immunofluorescence)
(AAA324449 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse NECAB3 Polyclonal Antibody | anti-NECAB3 antibody
NECAB3 Antibody
Gene Names
NECAB3; NIP1; XB51; STIP3; EFCBP3; SYTIP2; APBA2BP; dJ63M2.4; dJ63M2.5
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NECAB3, Antibody; NECAB3 Antibody; amyloid beta (A4) precursor protein binding, family A, member 2 binding protein; Amyloid beta A4 protein binding family A member 2 binding protein; Amyloid beta A4 protein-binding family A member 2-binding protein; APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; N terminal EF hand calcium binding protein 3; N-terminal EF-hand calcium-binding protein 3; NECA3_HUMAN; NECAB3; Nek2 interacting protein 1; Nek2-interacting protein 1; Neuronal calcium binding protein 3; Neuronal calcium-binding protein 3; NIP1; STIP3; Synaptotagmin interacting protein 2; SYTIP2; X11L binding protein 51; X11L-binding protein 51; XB51; anti-NECAB3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
NECAB3 antibody detects endogenous levels of total NECAB3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
362
Applicable Applications for anti-NECAB3 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Golgi Apparatus.
Tissue Specificity
Strongly expressed in heart and skeletal muscle, moderately in brain and pancreas.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog
Similarity
Pig (91%), Bovine (83%), Horse (91%), Rabbit (91%), Dog (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA324449 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA324449 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from COLO cells using NECAB3 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from COLO cells using NECAB3 antibody.)
Related Product Information for anti-NECAB3 antibody
Function: Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta. May enhance the activity of HIF1A and thus promote glycolysis under normoxic conditions; the function requires its ABM domain and may implicate the stabilization of the interaction between HIF1AN and APBA3.
Subunit Structure: Interacts with the N-terminal domain of APBA2. Interacts with NEK2. Interacts with APBA3; APBA3 seems to mediate the interaction between NECAB3 and HIF1AN.
Post-translational Modifications: Phosphorylated by NEK2.
Subunit Structure: Interacts with the N-terminal domain of APBA2. Interacts with NEK2. Interacts with APBA3; APBA3 seems to mediate the interaction between NECAB3 and HIF1AN.
Post-translational Modifications: Phosphorylated by NEK2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 44 kD
Predicted: 45 kDa
Predicted: 45 kDa
NCBI Official Full Name
N-terminal EF-hand calcium-binding protein 3 isoform 1
NCBI Official Synonym Full Names
N-terminal EF-hand calcium binding protein 3
NCBI Official Symbol
NECAB3
NCBI Official Synonym Symbols
NIP1; XB51; STIP3; EFCBP3; SYTIP2; APBA2BP; dJ63M2.4; dJ63M2.5
NCBI Protein Information
N-terminal EF-hand calcium-binding protein 3
UniProt Protein Name
N-terminal EF-hand calcium-binding protein 3
UniProt Gene Name
NECAB3
UniProt Synonym Gene Names
APBA2BP; NIP1; SYTIP2; XB51
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Product Notes
The NECAB3 necab3 (Catalog #AAA324449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NECAB3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NECAB3 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the NECAB3 necab3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NECAB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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