FCM/FACS (Flow Cytometry)
(NEDD4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit NEDD4 Polyclonal Antibody | anti-NEDD4 antibody
NEDD4 Antibody (C-term)
Gene Names
NEDD4; RPF1; NEDD4-1
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NEDD4, Antibody; NEDD4 Antibody (C-term); E3 ubiquitin-protein ligase NEDD4; 632-; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4; NEDD4; KIAA0093; NEDD4-1; anti-NEDD4 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NEDD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1288-1319 amino acids from the C-terminal region of human NEDD4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1288-1319
Sequence Length
900
Applicable Applications for anti-NEDD4 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Cellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein Note=Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1. {ECO:0000250, ECO:0000269|PubMed:18819914}
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(NEDD4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(NEDD4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(NEDD4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(NEDD4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of NEDD4 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of NEDD4 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.)
Related Product Information for anti-NEDD4 antibody
Nedd4 is one of a group of mouse genes that show developmentally regulated expression in mouse embryonic brain. Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.
Product Categories/Family for anti-NEDD4 antibody
References
Lin, Q., et al. Mol. Cell. Biol. 30(6):1541-1554(2010)
Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
Edwin, F., et al. J. Biol. Chem. 285(1):255-264(2010)
Guo, Y.Y., et al. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 31(6):679-685(2009)
Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
Edwin, F., et al. J. Biol. Chem. 285(1):255-264(2010)
Guo, Y.Y., et al. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 31(6):679-685(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
149114
NCBI Official Full Name
E3 ubiquitin-protein ligase NEDD4 isoform 1
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
NCBI Official Symbol
NEDD4
NCBI Official Synonym Symbols
RPF1; NEDD4-1
NCBI Protein Information
E3 ubiquitin-protein ligase NEDD4
UniProt Protein Name
E3 ubiquitin-protein ligase NEDD4
UniProt Gene Name
NEDD4
UniProt Synonym Gene Names
KIAA0093; NEDD4-1; NEDD-4
UniProt Entry Name
NEDD4_HUMAN
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Product Notes
The NEDD4 nedd4 (Catalog #AAA285343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1288-1319. The NEDD4 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NEDD4 nedd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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