IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA119291 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human, Mouse NEDD8 Polyclonal Antibody | anti-NEDD8 antibody
Rabbit anti-human NEDD8 polyclonal Antibody(NEDD8)
Gene Names
NEDD8; NEDD-8
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
NEDD8, Antibody; Rabbit anti-human NEDD8 polyclonal Antibody(NEDD8); NEDD8 (Neddylin) (Neural precursor cell expressed developmentally downregulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8), NEDD8; anti-NEDD8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Applicable Applications for anti-NEDD8 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human NEDD8 protein (1-81AA)
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Immunogen Species
Homo sapiens (Human)
Research Area
Cell Biology
Target Names
NEDD8
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA119291 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA119291 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA119291 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA119291 at dilution 1:100)
WB (Western Blot)
(All lanes: NEDD8 antibody at 16ug/mlLane 1:mouse heart tissueLane 2:mouse spleen tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 9kDaObserved band size: 9kDa)
WB (Western Blot)
(All lanes: NEDD8 antibody at 16ug/mlLane 1:mouse heart tissueLane 2:mouse spleen tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 9kDaObserved band size: 9kDa)
Related Product Information for anti-NEDD8 antibody
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
NEDD8
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol
NEDD8
NCBI Official Synonym Symbols
NEDD-8
NCBI Protein Information
NEDD8
UniProt Protein Name
NEDD8
UniProt Gene Name
NEDD8
UniProt Synonym Gene Names
NEDD-8
UniProt Entry Name
NEDD8_HUMAN
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Product Notes
The NEDD8 nedd8 (Catalog #AAA119291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human NEDD8 polyclonal Antibody(NEDD8) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD8 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NEDD8 nedd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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