IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using NEDD9 antibody. Blue: DAPI for nuclear staining.)
Rabbit NEDD9 Polyclonal Antibody | anti-NEDD9 antibody
NEDD9 Polyclonal Antibody
Gene Names
NEDD9; CAS2; CASL; HEF1; CAS-L; CASS2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
NEDD9, Antibody; NEDD9 Polyclonal Antibody; CAS2; CASL; HEF1; CAS-L; CASS2; anti-NEDD9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Concentration
1mg/ml (varies by lot)
Sequence Length
834
Applicable Applications for anti-NEDD9 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human NEDD9
Calculated Molecular Weight
92kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using NEDD9 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using NEDD9 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using NEDD9 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using NEDD9 antibody at dilution of 1:200 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using NEDD9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using NEDD9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-NEDD9 antibody
The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-NEDD9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
834
NCBI Official Full Name
Enhancer of filamentation 1
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 9
NCBI Official Symbol
NEDD9
NCBI Official Synonym Symbols
CAS2; CASL; HEF1; CAS-L; CASS2
NCBI Protein Information
enhancer of filamentation 1; cas-like docking; p130Cas-related protein; Enhancer of filamentation 1 p55; renal carcinoma antigen NY-REN-12; Cas scaffolding protein family member 2; Crk-associated substrate related protein Cas-L; neural precursor cell expressed developmentally down-regulated protein 9
UniProt Protein Name
Enhancer of filamentation 1
UniProt Gene Name
NEDD9
UniProt Synonym Gene Names
CASL; CASS2; hEF1; CAS-L; CasL; NEDD-9
UniProt Entry Name
CASL_HUMAN
Similar Products
Product Notes
The NEDD9 nedd9 (Catalog #AAA37426) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEDD9 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD9 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NEDD9 nedd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.