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ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-COBRA1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the TFF1 promoter.)
Rabbit anti-Human Negative elongation factor B Polyclonal Antibody | anti-NELFB antibody
Rabbit anti-human Negative elongation factor B polyclonal Antibody
Gene Names
NELFB; COBRA1; NELF-B
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Synonyms
Negative elongation factor B, Antibody; Rabbit anti-human Negative elongation factor B polyclonal Antibody; Cofactor of BRCA1; anti-NELFB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Sequence Length
628
Applicable Applications for anti-NELFB antibody
ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Negative elongation factor B protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-79991 / sc-79992
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-COBRA1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the TFF1 promoter.)
ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-COBRA1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the TFF1 promoter.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA81483 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA81483 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human endometrial cancer using AAA81483 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human endometrial cancer using AAA81483 at dilution 1:100)
WB (Western Blot)
(All lanes: Negative elongation factor B antibody at 2ug/mlLane 1: NIH3T3 whole cell lysateLane 2: HL-60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 64 kDaObserved band size: 64 kDa)
WB (Western Blot)
(All lanes: Negative elongation factor B antibody at 2ug/mlLane 1: NIH3T3 whole cell lysateLane 2: HL-60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 64 kDaObserved band size: 64 kDa)
WB (Western Blot)
(All lanes: Negative elongation factor B antibody at 2ug/mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 64 kDaObserved band size: 64 kDa)
WB (Western Blot)
(All lanes: Negative elongation factor B antibody at 2ug/mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 64 kDaObserved band size: 64 kDa)
Related Product Information for anti-NELFB antibody
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,697 Da
NCBI Official Full Name
negative elongation factor B
NCBI Official Synonym Full Names
negative elongation factor complex member B
NCBI Official Symbol
NELFB
NCBI Official Synonym Symbols
COBRA1; NELF-B
NCBI Protein Information
negative elongation factor B
UniProt Protein Name
Negative elongation factor B
UniProt Gene Name
NELFB
UniProt Synonym Gene Names
COBRA1; KIAA1182; NELF-B
UniProt Entry Name
NELFB_HUMAN
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Product Notes
The NELFB nelfb (Catalog #AAA81483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Negative elongation factor B polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Negative elongation factor B can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NELFB nelfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Negative elongation factor B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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