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WB (Western Blot)
(Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab.)
Rabbit anti-Human NEK2 Polyclonal Antibody | anti-NEK2 antibody
NEK2 Antibody (Center)
Gene Names
NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NEK2, Antibody; NEK2 Antibody (Center); Serine/threonine-protein kinase Nek2; HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1; NEK2; NEK2A; NLK1; anti-NEK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-426 amino acids from the Central region of human NEK2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
396-426
Sequence Length
388
Applicable Applications for anti-NEK2 antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
IF~~1:25
IHC~~1:100
WB~~1:1000
IHC~~1:100
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of lysates from 293T, Hela, Jurkat, K562, MCF-7, and mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410). AAA28694 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from 293T, Hela, Jurkat, K562, MCF-7, and mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410). AAA28694 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded M. brain section using NEK2 Antibody(Center). AAA28694 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded M. brain section using NEK2 Antibody(Center). AAA28694 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded R. brain section using NEK2 Antibody(Center). AAA28694 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded R. brain section using NEK2 Antibody(Center). AAA28694 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. brain section using NEK2 Antibody(Center). AAA28694 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. brain section using NEK2 Antibody(Center). AAA28694 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with hNEK2-C410. AAA28694 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with hNEK2-C410. AAA28694 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-NEK2 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-NEK2 antibody
References
Chen, Y., et al., J. Biol. Chem. 277(51):49408-49416 (2002). Eto, M., et al., J. Biol. Chem. 277(46):44013-44020 (2002). Schutte, B.C., et al., Genome Res. 10(1):81-94 (2000). Fry, A.M., et al., EMBO J. 17(2):470-481 (1998). Schultz, S.J., et al., Cell Growth Differ. 5(6):625-635 (1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51763
NCBI Official Full Name
serine/threonine-protein kinase Nek2 isoform 3
NCBI Official Synonym Full Names
NIMA-related kinase 2
NCBI Official Symbol
NEK2
NCBI Official Synonym Symbols
NLK1; RP67; NEK2A; HsPK21; PPP1R111
NCBI Protein Information
serine/threonine-protein kinase Nek2
UniProt Protein Name
Serine/threonine-protein kinase Nek2
UniProt Gene Name
NEK2
UniProt Synonym Gene Names
NEK2A; NLK1; NimA-related protein kinase 2
UniProt Entry Name
NEK2_HUMAN
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Product Notes
The NEK2 nek2 (Catalog #AAA28694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 396-426. The NEK2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC). IF~~1:25 IHC~~1:100 WB~~1:1000. Researchers should empirically determine the suitability of the NEK2 nek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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