IHC (Immunohistochemisry)
(Mouse Nek6 Antibody (C-term) (AAA287689)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nek6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit Nek6 Polyclonal Antibody | anti-Nek6 antibody
Mouse Nek6 Antibody (C-term)
Gene Names
Nek6; 1300007C09Rik
Reactivity
Human, mouse (Predicted Reactivity: Pig, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Nek6, Antibody; Mouse Nek6 Antibody (C-term); Serine/threonine-protein kinase Nek6; Never in mitosis A-related kinase 6; NimA-related protein kinase 6; Nek6; anti-Nek6 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Nek6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the C-terminal region of mouse Nek6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
251-280
Sequence Length
313
Applicable Applications for anti-Nek6 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Mouse Nek6 Antibody (C-term) (AAA287689)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nek6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Mouse Nek6 Antibody (C-term) (AAA287689)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nek6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of Nek6 (arrow) using rabbit polyclonal Mouse Nek6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek6 gene.)
WB (Western Blot)
(Western blot analysis of Nek6 (arrow) using rabbit polyclonal Mouse Nek6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek6 gene.)
WB (Western Blot)
(Mouse Nek6 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Nek6 antibody detected the Nek6 protein (arrow).)
WB (Western Blot)
(Mouse Nek6 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Nek6 antibody detected the Nek6 protein (arrow).)
Related Product Information for anti-Nek6 antibody
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis (By similarity).
Product Categories/Family for anti-Nek6 antibody
References
Jeon, Y.J., et al. J. Biol. Chem. 285(36):28126-28133(2010)
Kaput, J., et al. Physiol. Genomics 18(3):316-324(2004)
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Hashimoto, Y., et al. Biochem. Biophys. Res. Commun. 293(2):753-758(2002)
Feige, E., et al. Mech. Dev. 110 (1-2), 219-223 (2002) :
Kaput, J., et al. Physiol. Genomics 18(3):316-324(2004)
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Hashimoto, Y., et al. Biochem. Biophys. Res. Commun. 293(2):753-758(2002)
Feige, E., et al. Mech. Dev. 110 (1-2), 219-223 (2002) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35742
NCBI Official Full Name
serine/threonine-protein kinase Nek6
NCBI Official Synonym Full Names
NIMA (never in mitosis gene a)-related expressed kinase 6
NCBI Official Symbol
Nek6
NCBI Official Synonym Symbols
1300007C09Rik
NCBI Protein Information
serine/threonine-protein kinase Nek6
UniProt Protein Name
Serine/threonine-protein kinase Nek6
UniProt Gene Name
Nek6
UniProt Synonym Gene Names
NimA-related protein kinase 6
UniProt Entry Name
NEK6_MOUSE
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Product Notes
The Nek6 nek6 (Catalog #AAA287689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 251-280. The Mouse Nek6 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Nek6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Nek6 nek6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nek6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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