WB (Western Blot)
(Detection of human NEK7 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NEK7 antibody AAA211965 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human NEK7 Polyclonal Antibody | anti-NEK7 antibody
Rabbit anti-NEK7 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NEK7, Antibody; Rabbit anti-NEK7 Antibody, Affinity Purified; serine/threonine-protein kinase Nek7; nimA-related protein kinase 7; NIMA (never in mitosis gene a)-related kinase 7; never in mitosis A-related kinase 7; Serine/threonine-protein kinase Nek7; NIMA related kinase 7; anti-NEK7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NEK7 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NEK7 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NEK7 antibody AAA211965 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human NEK7 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NEK7 antibody AAA211965 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human NEK7 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NEK7 (Cat. No. AAA211965 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NEK7 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NEK7 (Cat. No. AAA211965 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-NEK7 antibody
NEK7 [NIMA(never in mitosis gene a)-related kinase 7] is a member of the NIMA-related serine/threonine kinase family. NEK7 has been shown localize to spindle poles and to be required for mitotic spindle formation and cytokinesis.
Product Categories/Family for anti-NEK7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,551 Da
NCBI Official Full Name
serine/threonine-protein kinase Nek7
NCBI Official Synonym Full Names
NIMA-related kinase 7
NCBI Official Symbol
NEK7
NCBI Protein Information
serine/threonine-protein kinase Nek7; nimA-related protein kinase 7; never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7
UniProt Protein Name
Serine/threonine-protein kinase Nek7
UniProt Gene Name
NEK7
UniProt Synonym Gene Names
NimA-related protein kinase 7
UniProt Entry Name
NEK7_HUMAN
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Product Notes
The NEK7 nek7 (Catalog #AAA211965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NEK7 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK7 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NEK7 nek7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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