Rabbit NELF-A Polyclonal Antibody | anti-NELF-A antibody

NELF-A Antibody

Cat. Num. AAA328614

• Gene Names: NELFA; WHSC2; NELF-A; P/OKcl.15
• Reactivity: Human, Mouse; Predicted: Bovine
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NELF-A, Antibody; NELF-A Antibody; FLJ10442; FLJ25112; Negative elongation factor A; Negative elongation factor polypeptide A; NELF A; NELF-A; NELFA; NELFA_HUMAN; P/OKcl.15; P/OKcl15; WHSC 2; Whsc2; Wolf Hirschhorn syndrome candidate 2; Wolf Hirschhorn syndrome candidate 2 protein; Wolf-Hirschhorn syndrome candidate 2 protein; anti-NELF-A antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NELF-A Antibody detects endogenous levels of NELF-A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 528

• Applicable Applications for anti-NELF-A antibody: WB (Western Blot)
• Immunogen: A synthesized peptide derived from human NELF-A.
• Tissue Specificity: Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts from Jurkat cells?, using NELF-A Antibody. The lane on the left was treated with blocking peptide.)

WB (Western Blot)

(Western blot analysis of extracts from 293, using NELF-A Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-NELF-A antibody

Description: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner. Probably required to interact with the RNA polymerase II complex.

Subunit Structure: The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; NELFA and NELFCD form a stable subcomplex that binds to the N-terminus of NELFB. Interacts with the RNA polymerase II complex when it is not phosphorylated by P-TEFb.

Similarity: The HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex. Belongs to the NELF-A family.

NCBI and Uniprot Product Information

• NCBI GI #: 212276499
• NCBI GeneID: 7469
• UniProt Accession #: Q9H3P2
• Molecular Weight: Observed: 58 kDa; Predicted: 58 kDa
• NCBI Official Full Name: Negative elongation factor A
• NCBI Official Synonym Full Names: negative elongation factor complex member A
• NCBI Official Symbol: NELFA
• NCBI Official Synonym Symbols: WHSC2; NELF-A; P/OKcl.15
• NCBI Protein Information: negative elongation factor A
• UniProt Protein Name: Negative elongation factor A
• UniProt Gene Name: NELFA
• UniProt Synonym Gene Names: WHSC2; NELF-A
• UniProt Entry Name: NELFA_HUMAN