IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using NELFA Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
Rabbit anti-Human, Mouse NELFA Polyclonal Antibody | anti-NELFA antibody
NELFA Antibody
Gene Names
NELFA; WHSC2; NELF-A; P/OKcl.15
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
NELFA, Antibody; NELFA Antibody; WHSC2; NELF-A; P/OKcl.15; anti-NELFA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total NELFA protein.
Purity/Purification
Antigen affinity purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.2mg/ml (varies by lot)
Sequence Length
539
Applicable Applications for anti-NELFA antibody
WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Fusion protein of human NELFA
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C
IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using NELFA Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using NELFA Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using NELFA Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using NELFA Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
SDS-PAGE
(Gel: 8%SDS-PAGE Lysate: 40 ug, Lane 1-5: Hela£¬RAW264.7£¬NIH/3T3£¬Jurkat cell lysates and Mouse testis tissue lysate, Primary antibody:NELFA antibody at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
SDS-PAGE
(Gel: 8%SDS-PAGE Lysate: 40 ug, Lane 1-5: Hela£¬RAW264.7£¬NIH/3T3£¬Jurkat cell lysates and Mouse testis tissue lysate, Primary antibody:NELFA antibody at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
Related Product Information for anti-NELFA antibody
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.
Product Categories/Family for anti-NELFA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,416 Da
NCBI Official Full Name
negative elongation factor A
NCBI Official Synonym Full Names
negative elongation factor complex member A
NCBI Official Symbol
NELFA
NCBI Official Synonym Symbols
WHSC2; NELF-A; P/OKcl.15
NCBI Protein Information
negative elongation factor A
UniProt Protein Name
Negative elongation factor A
UniProt Gene Name
NELFA
UniProt Synonym Gene Names
WHSC2; NELF-A
UniProt Entry Name
NELFA_HUMAN
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Product Notes
The NELFA nelfa (Catalog #AAA309205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NELFA Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NELFA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NELFA nelfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NELFA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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