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product-image-AAA211643_WB13.jpg WB (Western Blot) (Detection of human and mouse NF-kappaB2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF-kappaB2 antibody AAA211643 (lot AAA211643-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

Rabbit anti-Human, Mouse NF-kappaB2 Polyclonal Antibody | anti-NF-kappaB2 antibody

Rabbit anti-NF-kappaB2 Antibody, Affinity Purified

Gene Names
NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NF-kappaB2, Antibody; Rabbit anti-NF-kappaB2 Antibody, Affinity Purified; transcription factor NFKB2; nuclear factor of Kappa light chain gene enhancer in B cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); oncogene Lyt-10; p100; p52; nuclear factor NF-kappa-B p100 subunit; nuclear factor NF-kappa-B p52 subunit; p49/p100; NF-kB2; NFKB, p52/p100 subunit; LYT-10; LYT10; lymphocyte translocation chromosome 10 protein; H2TF1; DNA-binding factor KBF2; CVID10; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; nuclear factor Kappa-B, subunit 2; Nuclear factor NF-kappa-B p100 subunit; nuclear factor kappa B subunit 2; anti-NF-kappaB2 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-NF-kappaB2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 850 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse NF-kappaB2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF-kappaB2 antibody AAA211643 (lot AAA211643-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA211643_WB13.jpg WB (Western Blot) (Detection of human and mouse NF-kappaB2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF-kappaB2 antibody AAA211643 (lot AAA211643-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation)

(Detection of human NF-kappaB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF-kappaB2 antibody AAA211643 (lot AAA211643-2) used for IP at 6 ug per reaction. NF-kappaB2 was also immunoprecipitated by rabbit anti-NF-kappaB2 antibody BL7042. For blotting immunoprecipitated NF-kappaB2, AAA211643 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA211643_IP15.jpg IP (Immunoprecipitation) (Detection of human NF-kappaB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF-kappaB2 antibody AAA211643 (lot AAA211643-2) used for IP at 6 ug per reaction. NF-kappaB2 was also immunoprecipitated by rabbit anti-NF-kappaB2 antibody BL7042. For blotting immunoprecipitated NF-kappaB2, AAA211643 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-NF-kappaB2 antibody
The NFKB2 gene encodes a 100 kDa protein that can undergo co-translational processing to also yield a 52 kDa protein. The 52 kDa protein is also known as NF-kappa B2/p52 and is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. The 100 kDa product is also known as p100 or I kappa B delta. P100/I kappa B delta has been shown to function as an inhibitor protein that interacts with NF-kappa B and inhibit its DNA binding activity. P100/I kappa B delta is a member of the I kappa B family of proteins which includes I kappa B-beta, I kappa B-delta/NF-kappa B2/p100, I kappa B-gamma/NF-kappa B1/p105, I kappa B-epsilon, I kappa B-zeta, and Bcl3. The classical description of the NF-kappa B transcription factor is a transcription factor that reprograms gene expression of the immune system in response to injury, infection, and stress.
Product Categories/Family for anti-NF-kappaB2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
96,678 Da
NCBI Official Full Name
nuclear factor NF-kappa-B p100 subunit isoform b
NCBI Official Synonym Full Names
nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
NCBI Official Symbol
NFKB2
NCBI Official Synonym Symbols
p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
NCBI Protein Information
nuclear factor NF-kappa-B p100 subunit; oncogene Lyt-10; NFKB, p52/p100 subunit; DNA-binding factor KBF2; transcription factor NFKB2; nuclear factor Kappa-B, subunit 2; nuclear factor NF-kappa-B p52 subunit; lymphocyte translocation chromosome 10 protein;
UniProt Protein Name
Nuclear factor NF-kappa-B p100 subunit
UniProt Gene Name
NFKB2
UniProt Synonym Gene Names
LYT10; Lyt10
UniProt Entry Name
NFKB2_HUMAN

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Product Notes

The NF-kappaB2 nfkb2 (Catalog #AAA211643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NF-kappaB2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NF-kappaB2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NF-kappaB2 nfkb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-kappaB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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