IP (Immunoprecipitation)
(NEFM was immunoprecipitated using:; Lane A:0.5 mg 293T Whole Cell Lysate; 4 uL anti-NEFM rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-NEFM rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 150 kDa; Observed band size :150 kDa)
Rabbit anti-Human NF-M/NEFM Polyclonal Antibody | anti-NF-M/NEFM antibody
NF-M/NEFM Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
NF-M/NEFM, Antibody; NF-M/NEFM Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-NEF3 Antibody; Anti-NF-M Antibody; Anti-NFM Antibody; anti-NF-M/NEFM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human NF-M/NEFM
Applicable Applications for anti-NF-M/NEFM antibody
IP (Immunoprecipitation), WB (Western Blot)
Preparation
Produced in rabbits immunized with E Coli-derived Human NF-M/NEFM fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(NEFM was immunoprecipitated using:; Lane A:0.5 mg 293T Whole Cell Lysate; 4 uL anti-NEFM rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-NEFM rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 150 kDa; Observed band size :150 kDa)
IP (Immunoprecipitation)
(NEFM was immunoprecipitated using:; Lane A:0.5 mg 293T Whole Cell Lysate; 4 uL anti-NEFM rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-NEFM rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 150 kDa; Observed band size :150 kDa)
WB (Western Blot)
(Anti-NF-M/NEFM rabbit polyclonal antibody at 1:500 dilution; Lane A: 293T Whole Cell Lysate; Lane B: SH-SY5Y Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:102 kDa)
WB (Western Blot)
(Anti-NF-M/NEFM rabbit polyclonal antibody at 1:500 dilution; Lane A: 293T Whole Cell Lysate; Lane B: SH-SY5Y Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:102 kDa)
Related Product Information for anti-NF-M/NEFM antibody
NEFM (Neurofilament Medium) is a Protein Coding gene. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity). Alternative splicing results in multiple transcript variants encoding distinct isoforms. Diseases associated with NEFM include Charcot-Marie-Tooth Disease, Demyelinating, Type 1F, and Charcot-Marie-Tooth Disease, Axonal, Type 2E. Among its related pathways are Neuroscience and Association Between Physico-Chemical Features and Toxicity Associated Pathways.
Product Categories/Family for anti-NF-M/NEFM antibody
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Product Notes
The NF-M/NEFM (Catalog #AAA258677) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NF-M/NEFM Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NF-M/NEFM can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NF-M/NEFM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-M/NEFM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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