Rabbit anti-Human, Mouse NF-YA Polyclonal Antibody | anti-NF-YA antibody

Rabbit anti-NF-YA Antibody, Affinity Purified

Cat. Num. AAA211769

• Gene Names: NFYA; HAP2; CBF-A; CBF-B; NF-YA
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: NF-YA, Antibody; Rabbit anti-NF-YA Antibody, Affinity Purified; HAP2 CCAAT-binding protein; HAP2; Transcription factor NF-Y, A subunit; nuclear transcription factor Y, alpha; nuclear transcription factor Y subunit alpha; nuclear transcription factor Y subunit A; CAAT box DNA-binding protein subunit A; CCAAT-binding transcription factor subunit B; CBF-B; CBF-A; CAAT-box DNA binding protein subunit A; NF-YA; Nuclear transcription factor Y subunit alpha; anti-NF-YA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-NF-YA antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Predicted Reactivity: Rat, Bovine
• Immunogen: Between 297 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse NF-YA by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibody: Affinity purified rabbit anti-NF-YA antibody AAA211769 (lot AAA211769-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human NF-YA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-YA antibody AAA211769 (lot AAA211769-1) used for IP at 3 ug/mg lysate. NF-YA was also immunoprecipitated by rabbit anti-NF-YA antibody which recognizes an upstream epitope. For blotting immunoprecipitated NF-YA, AAA211769 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure timesof 5 seconds.)

IHC (Immunohistochemistry)

(Detection of human and mouse NF-YA by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-NF-YA (Cat. No. AAA211769 lot 1) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)

Related Product Information for anti-NF-YA antibody

Nuclear transcription factor Y alpha (NF-YA) is a subunit of the NF-Y heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC. The NF-Y factor binds the CCAAT box with high specificity and affinity, and it is the major CCAAT-binding activator. All 3 subunits are essential for CCAAT binding. NF-Y plays a role in regulating several cell-cycle regulated genes such as CDC2, CDC25A/B/C, cyclin A2, cyclin B1/B2, and E2F1.

Product Categories/Family for anti-NF-YA antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 11496974
• NCBI GeneID: 4800
• NCBI Accession #: NP_068351.1
• NCBI GenBank Nucleotide #: NM_021705.3
• UniProt Accession #: P23511; Q8IXU0
• Molecular Weight: 33,939 Da
• NCBI Official Full Name: nuclear transcription factor Y subunit alpha isoform 2
• NCBI Official Synonym Full Names: nuclear transcription factor Y, alpha
• NCBI Official Symbol: NFYA
• NCBI Official Synonym Symbols: HAP2; CBF-A; CBF-B; NF-YA
• NCBI Protein Information: nuclear transcription factor Y subunit alpha; HAP2 CCAAT-binding protein; Transcription factor NF-Y, A subunit; CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; nuclear transcription factor Y subunit A; CCAAT-binding transcr
• UniProt Protein Name: Nuclear transcription factor Y subunit alpha
• UniProt Gene Name: NFYA
• UniProt Synonym Gene Names: NF-YA
• UniProt Entry Name: NFYA_HUMAN