IP (Immunoprecipitation)
(Detection of human NF-YC by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-YC antibody AAA211770 used for IP at 3 ug/mg lysate. NF-YC was also immunoprecipitated by rabbit anti-NF-YC antibody BL8376, which recognizes an upstream epitope. For blotting immunoprecipitated NF-YC, BL8376 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Human NF-YC Polyclonal Antibody | anti-NF-YC antibody
Rabbit anti-NF-YC Antibody, Affinity Purified
Gene Names
NFYC; HSM; CBFC; HAP5; CBF-C; NF-YC; H1TF2A
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
NF-YC, Antibody; Rabbit anti-NF-YC Antibody, Affinity Purified; CAAT box DNA-binding protein subunit C; NF-YC; transcription factor NF-Y, C subunit; transactivator HSM-1/2; transactivator HSM-1; nuclear transcription factor Y, gamma; nuclear transcription factor Y subunit gamma; nuclear transcription factor Y subunit C; HSM; histone H1 transcription factor large subunit 2A; HAP5; H1TF2A; CCAAT transcription binding factor subunit gamma; CCAAT binding factor subunit C; CBFC; CBF-C; Nuclear transcription factor Y subunit gamma; anti-NF-YC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NF-YC antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Bovine, Orangutan
Immunogen
Between 408 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human NF-YC by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-YC antibody AAA211770 used for IP at 3 ug/mg lysate. NF-YC was also immunoprecipitated by rabbit anti-NF-YC antibody BL8376, which recognizes an upstream epitope. For blotting immunoprecipitated NF-YC, BL8376 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human NF-YC by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-YC antibody AAA211770 used for IP at 3 ug/mg lysate. NF-YC was also immunoprecipitated by rabbit anti-NF-YC antibody BL8376, which recognizes an upstream epitope. For blotting immunoprecipitated NF-YC, BL8376 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemistry)
(Detection of human NF-YC by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-NF-YC (Cat. No. AAA211770 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NF-YC by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-NF-YC (Cat. No. AAA211770 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-NF-YC antibody
Nuclear transcription factor Y gamma (NF-YC) is a subunit of the NF-Y heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC. The NF-Y factor binds the CCAAT box with high specificity and affinity, and it is the major CCAAT-binding activator. All 3 subunits are essential for CCAAT binding. NF-Y plays a role in regulating several cell-cycle regulated genes such as CDC2, CDC25A/B/C, cyclin A2, cyclin B1/B2, and E2F1.
Product Categories/Family for anti-NF-YC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,302 Da
NCBI Official Full Name
nuclear transcription factor Y subunit gamma isoform 2
NCBI Official Synonym Full Names
nuclear transcription factor Y, gamma
NCBI Official Symbol
NFYC
NCBI Official Synonym Symbols
HSM; CBFC; HAP5; CBF-C; NF-YC; H1TF2A
NCBI Protein Information
nuclear transcription factor Y subunit gamma; transactivator HSM-1; transactivator HSM-1/2; CCAAT binding factor subunit C; transcription factor NF-Y, C subunit; CAAT box DNA-binding protein subunit C; nuclear transcription factor Y subunit C; CCAAT trans
UniProt Protein Name
Nuclear transcription factor Y subunit gamma
UniProt Gene Name
NFYC
UniProt Synonym Gene Names
NF-YC
UniProt Entry Name
NFYC_HUMAN
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Product Notes
The NF-YC nfyc (Catalog #AAA211770) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NF-YC Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NF-YC can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the NF-YC nfyc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-YC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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