WB (Western Blot)
(Detection of human and mouse NF45 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NF45 antibody AAA212126 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
Rabbit anti-Human, Mouse NF45 Polyclonal Antibody | anti-NF45 antibody
Rabbit anti-NF45 Antibody, Affinity Purified
Gene Names
ILF2; NF45; PRO3063
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NF45, Antibody; Rabbit anti-NF45 Antibody, Affinity Purified; interleukin enhancer binding factor 2, 45kDa; PRO3063; nuclear factor of activated T-cells, 45-kDa; Nuclear factor of activated T-cells 45 kDa; interleukin enhancer binding factor 2, 45kD; NF45; interleukin enhancer-binding factor 2; Interleukin enhancer-binding factor 2; interleukin enhancer binding factor 2; anti-NF45 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NF45 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, X. tropicalis, Orangutan
Immunogen
Between 340 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NF45 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NF45 antibody AAA212126 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
WB (Western Blot)
(Detection of human and mouse NF45 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NF45 antibody AAA212126 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
IHC (Immunohiostchemistry)
(Detection of human NF45 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-NF45 (Cat. No. AAA212126 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohiostchemistry)
(Detection of human NF45 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-NF45 (Cat. No. AAA212126 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of mouse NF45 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NF45 (Cat. No. AAA212126 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse NF45 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NF45 (Cat. No. AAA212126 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-NF45 antibody
The 45kDa nuclear factor of activated T-cells (NF45) functions as part of a heterodimeric complex with NF90 to form the transcription factor, NFAT (nuclear factor of activated T-cells). The NF45/NF90 complex binds the interleukin-2 promoter and regulates transcription of the IL2 gene during T-cell activation. It has also been shown that the NF45/NF90 complex can function as a negative regulator of miRNA biogenesis.
Product Categories/Family for anti-NF45 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,062 Da
NCBI Official Full Name
interleukin enhancer-binding factor 2 isoform 1
NCBI Official Synonym Full Names
interleukin enhancer binding factor 2
NCBI Official Symbol
ILF2
NCBI Official Synonym Symbols
NF45; PRO3063
NCBI Protein Information
interleukin enhancer-binding factor 2; interleukin enhancer binding factor 2, 45kD; nuclear factor of activated T-cells, 45-kDa; interleukin enhancer binding factor 2, 45kDa
UniProt Protein Name
Interleukin enhancer-binding factor 2
UniProt Gene Name
ILF2
UniProt Synonym Gene Names
NF45
UniProt Entry Name
ILF2_HUMAN
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Product Notes
The NF45 ilf2 (Catalog #AAA212126) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NF45 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NF45 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NF45 ilf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF45, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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