WB (Western Blot)
(Detection of human NF90 and NF110 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NF90/NF110 antibody AAA212116 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies and as well as anti-NF110 antibody each of which recognizes a different epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).)
Rabbit anti-Human NF90/NF110 Polyclonal Antibody | anti-NF90/NF110 antibody
Rabbit anti-NF90/NF110 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NF90/NF110, Antibody; Rabbit anti-NF90/NF110 Antibody, Affinity Purified; nuclear factor associated with dsRNA; NF110b; NF90a; NF90b; NFAR; NFAR-1; NF-AT-90; NF110; nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; TCP110; TCP80; translational control protein 80; NFAR2; DRBP76; CBTF; Double-stranded RNA-binding protein 76; NF90; DRBF; MPP4; dsRNA binding protein NFAR-2/MPP4; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; interleukin enhancer-binding factor 3; MMP4; M-phase phosphoprotein 4; MPHOSPH4; double-stranded RNA-binding protein, 76 kD; Interleukin enhancer-binding factor 3; interleukin enhancer binding factor 3; anti-NF90/NF110 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-NF90/NF110 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NF90 and NF110 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NF90/NF110 antibody AAA212116 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies and as well as anti-NF110 antibody each of which recognizes a different epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).)
WB (Western Blot)
(Detection of human NF90 and NF110 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NF90/NF110 antibody AAA212116 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies and as well as anti-NF110 antibody each of which recognizes a different epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).)
IHC (Immunohistochemistry)
(Detection of human NF90 and NF110 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NF90/NF110 (Cat. No. Lot1, upper panel, Cat. No. AAA212116 Lot1, middle panel and Cat. No. Lot1, lower panel) used at a dilution of 1:5,000 (0.2ug/ml) or 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NF90 and NF110 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NF90/NF110 (Cat. No. Lot1, upper panel, Cat. No. AAA212116 Lot1, middle panel and Cat. No. Lot1, lower panel) used at a dilution of 1:5,000 (0.2ug/ml) or 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-NF90/NF110 antibody
NFAR (nuclear factor associated with dsRNA) exists as two major protein species of 90 kDa (NFAR-1 or NF90) and 110 kDa (NFAR-2 or NF110) in the cell. NF90 and NF110 arise from a single gene named interleukin enhancer binding factor (ILF3) gene which resides on chromosome 19p13 and are generated through alternative splicing events. NF90 is a dsRNA binding protein that stabilizes mRNAs and regulates transcription. NF90 is a subunit of the nuclear factor of activated T-cells (NFAT) transcription factor that is required for T-cell expression of interleukin 2. The NF110 isoform is restricted to the nucleus and its function may be unrelated to NF90.
Product Categories/Family for anti-NF90/NF110 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NF90/NF110 ilf3 (Catalog #AAA212116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NF90/NF110 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NF90/NF110 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NF90/NF110 ilf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF90/NF110, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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