Rabbit anti-Human NFAT5 Polyclonal Antibody | anti-NFAT5 antibody

Rabbit anti-NFAT5 Antibody, Affinity Purified

Cat. Num. AAA213133

• Gene Names: NFAT5; NFATZ; OREBP; NF-AT5; NFATL1; TONEBP; KIAA0827
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: NFAT5, Antibody; Rabbit anti-NFAT5 Antibody, Affinity Purified; nuclear factor of activated T-cells 5, tonicity-responsive; TonE-binding protein; TONEBP; T-cell transcription factor NFAT5; osmotic response element-binding protein; OREBP; NFATZ; NFAT-like protein 1; NFATL1; glutamine rich protein H65; NF-AT5; nuclear factor of activated T-cells 5; tonicity-responsive enhancer-binding protein; Nuclear factor of activated T-cells 5; nuclear factor of activated T cells 5; anti-NFAT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-NFAT5 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Predicted Reactivity: Mouse, Rat
• Immunogen: Between 1481 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human NFAT5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NFAT5 antibody AAA213133 (lot AAA213133-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human NFAT5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NFAT5 antibody AAA213133 (lot AAA213133-1) used for IP at 6 ug per reaction. NFAT5 was also immunoprecipitated by rabbit anti-NFAT5 antibody For blotting immunoprecipitated NFAT5, AAA213133 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

Related Product Information for anti-NFAT5 antibody

The product of the NFAT5 (TONEBP) gene, nuclear factor of activated T-cells 5, is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. NFAT5 regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, NFAT5 exists as a homodimer and forms stable dimers with DNA elements [taken from NCBI Entrez Gene (Gene ID: 10725)].

Product Categories/Family for anti-NFAT5 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 27886520
• NCBI GeneID: 10725
• NCBI Accession #: NP_619728.2
• NCBI GenBank Nucleotide #: NM_138714.3
• UniProt Accession #: O94916; Q7LA65; Q96QH3; Q9UE63
• Molecular Weight: 157,966 Da
• NCBI Official Full Name: nuclear factor of activated T-cells 5 isoform a
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells 5, tonicity-responsive
• NCBI Official Symbol: NFAT5
• NCBI Official Synonym Symbols: NFATZ; OREBP; NF-AT5; NFATL1; TONEBP; KIAA0827
• NCBI Protein Information: nuclear factor of activated T-cells 5; OTTHUMP00000174893; OTTHUMP00000174894; NFAT-like protein 1; TonE-binding protein; glutamine rich protein H65; T-cell transcription factor NFAT5; osmotic response element-binding protein; tonicity-responsive enhancer
• UniProt Protein Name: Nuclear factor of activated T-cells 5
• UniProt Gene Name: NFAT5
• UniProt Synonym Gene Names: KIAA0827; TONEBP; NF-AT5; TonE-binding protein; TonEBP
• UniProt Entry Name: NFAT5_HUMAN