IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with NFATC4 Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFATC4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFATC4 immunoreactivity is localized to Cytoplasm significantly.)
Rabbit anti-Human NFATC4 Polyclonal Antibody | anti-NFATC4 antibody
NFATC4 Antibody (C-term)
Gene Names
NFATC4; NFAT3; NF-AT3; NF-ATC4
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NFATC4, Antibody; NFATC4 Antibody (C-term); Nuclear factor of activated T-cells; cytoplasmic 4; NF-ATc4; NFATc4; T-cell transcription factor NFAT3; NF-AT3; NFATC4; NFAT3; anti-NFATC4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NFATC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744-773 amino acids from the C-terminal region of human NFATC4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
744-773
Sequence Length
964
Applicable Applications for anti-NFATC4 antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with NFATC4 Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFATC4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFATC4 immunoreactivity is localized to Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with NFATC4 Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFATC4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFATC4 immunoreactivity is localized to Cytoplasm significantly.)
WB (Western Blot)
(NFATC4 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NFATC4 antibody detected the NFATC4 protein (arrow).)
WB (Western Blot)
(NFATC4 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NFATC4 antibody detected the NFATC4 protein (arrow).)
Related Product Information for anti-NFATC4 antibody
The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq].
Product Categories/Family for anti-NFATC4 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Al-Daraji, W.I., et al. J. Cutan. Pathol. 37 (9), E21-E36 (2010) : Fougere, M., et al. Oncogene 29(15):2292-2301(2010) Davila, S., et al. Genes Immun. 11(3):232-238(2010) Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95449
NCBI Official Full Name
nuclear factor of activated T-cells, cytoplasmic 4 isoform 1
NCBI Official Synonym Full Names
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
NCBI Official Symbol
NFATC4
NCBI Official Synonym Symbols
NFAT3; NF-AT3; NF-ATC4
NCBI Protein Information
nuclear factor of activated T-cells, cytoplasmic 4
UniProt Protein Name
Nuclear factor of activated T-cells, cytoplasmic 4
UniProt Gene Name
NFATC4
UniProt Synonym Gene Names
NFAT3; NF-ATc4; NFATc4; NF-AT3
UniProt Entry Name
NFAC4_HUMAN
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Product Notes
The NFATC4 nfatc4 (Catalog #AAA286532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 744-773. The NFATC4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFATC4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the NFATC4 nfatc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFATC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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