IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human, Mouse NFIL3 Polyclonal Antibody | anti-NFIL3 antibody
NFIL3 Polyclonal Antibody
Gene Names
NFIL3; E4BP4; IL3BP1; NFIL3A; NF-IL3A
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purification
Synonyms
NFIL3, Antibody; NFIL3 Polyclonal Antibody; E4BP4; IL3BP1; NF-IL3A; NFIL3A; anti-NFIL3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
AYAQEIQKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHSPQSSLSDVSEVSSVEHTQESSVQGSCRSPENKFQIIKQEPMELESYTREPRDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDGVVGKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEFEATQKLSSPIDMTSKRHFELEKHSAPSMVHSSLTPFSVQVTN
Sequence Length
462
Applicable Applications for anti-NFIL3 antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant protein of human NFIL3
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of mouse liver, using NFIL3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of mouse liver, using NFIL3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
Related Product Information for anti-NFIL3 antibody
The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene.
Product Categories/Family for anti-NFIL3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 51kDa
Observed: 55kDa
Observed: 55kDa
NCBI Official Full Name
nuclear factor interleukin-3-regulated protein
NCBI Official Synonym Full Names
nuclear factor, interleukin 3 regulated
NCBI Official Symbol
NFIL3
NCBI Official Synonym Symbols
E4BP4; IL3BP1; NFIL3A; NF-IL3A
NCBI Protein Information
nuclear factor interleukin-3-regulated protein
UniProt Protein Name
Nuclear factor interleukin-3-regulated protein
UniProt Gene Name
NFIL3
UniProt Synonym Gene Names
E4BP4; IL3BP1
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Product Notes
The NFIL3 nfil3 (Catalog #AAA281181) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFIL3 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NFIL3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the NFIL3 nfil3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AYAQEIQKLS NSTAVYFQDY QTSKSNVSSF VDEHEPSMVS SSCISVIKHS PQSSLSDVSE VSSVEHTQES SVQGSCRSPE NKFQIIKQEP MELESYTREP RDDRGSYTAS IYQNYMGNSF SGYSHSPPLL QVNRSSSNSP RTSETDDGVV GKSSDGEDEQ QVPKGPIHSP VELKHVHATV VKVPEVNSSA LPHKLRIKAK AMQIKVEAFD NEFEATQKLS SPIDMTSKRH FELEKHSAPS MVHSSLTPFS VQVTN. It is sometimes possible for the material contained within the vial of "NFIL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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