WB (Western Blot)
(NFkB p65 (pAb) tested by Western blot. NFkB p65 detection by Western blot. The analysis was performed using 20 ug HeLa cytoplasmic extract and NFkB p65 pAb at a 1:2,500 dilution.)
Rabbit anti-Human NFkB p65 Polyclonal Antibody | anti-NFkBp65 antibody
NFkB p65 antibody (pAb)
Gene Names
RELA; p65; CMCU; NFKB3
Reactivity
Human
Applications
Western Blot, Gel Super Shift Assay, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Synonyms
NFkB p65, Antibody; NFkB p65 antibody (pAb); RELA; p65; NFKB3; anti-NFkBp65 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-NFkBp65 antibody
WB (Western Blot), EMSA (Electrophoretic Mobility Shift Assay), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This NFkB p65 antibody was raised against a peptide immunogen corresponding to the C-terminal of human p65.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(NFkB p65 (pAb) tested by Western blot. NFkB p65 detection by Western blot. The analysis was performed using 20 ug HeLa cytoplasmic extract and NFkB p65 pAb at a 1:2,500 dilution.)
WB (Western Blot)
(NFkB p65 (pAb) tested by Western blot. NFkB p65 detection by Western blot. The analysis was performed using 20 ug HeLa cytoplasmic extract and NFkB p65 pAb at a 1:2,500 dilution.)
ChIP (Chromatin Immunoprecipitation)
(NFkB p65 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 2 million HT29 cells treated with TNF and 10 ul of p65 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(NFkB p65 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 2 million HT29 cells treated with TNF and 10 ul of p65 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(NFkB p65 antibody tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 25 ug of chromatin from a human brain tumor and 10 ul of NFkB p65 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.5 million sequence tags were mapped to identify NFkB p65 binding sites on chromosome 19.)
ChIP (Chromatin Immunoprecipitation)
(NFkB p65 antibody tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 25 ug of chromatin from a human brain tumor and 10 ul of NFkB p65 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.5 million sequence tags were mapped to identify NFkB p65 binding sites on chromosome 19.)
Related Product Information for anti-NFkBp65 antibody
Short Description: Our NFkB p65 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Supershift and Western blot; it has been shown to react with Human samples.
Background: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.
Background: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.
Product Categories/Family for anti-NFkBp65 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60kDa
NCBI Official Full Name
transcription factor p65 isoform 1
NCBI Official Synonym Full Names
RELA proto-oncogene, NF-kB subunit
NCBI Official Symbol
RELA
NCBI Official Synonym Symbols
p65; CMCU; NFKB3
NCBI Protein Information
transcription factor p65
UniProt Protein Name
Transcription factor p65
UniProt Gene Name
RELA
UniProt Synonym Gene Names
NFKB3
UniProt Entry Name
TF65_HUMAN
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Product Notes
The NFkBp65 rela (Catalog #AAA59863) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFkB p65 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB p65 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), EMSA (Electrophoretic Mobility Shift Assay), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the NFkBp65 rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB p65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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