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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NFKBIA Antibody (S32/36) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Rabbit NFKBIA Polyclonal Antibody | anti-NFKBIA antibody
NFKBIA Antibody (S32/36)
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human (Predicted Reactivity: Mouse, Pig)
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NFKBIA, Antibody; NFKBIA Antibody (S32/36); NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI; anti-NFKBIA antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from human NFKBIA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
12-41
Sequence Length
317
Applicable Applications for anti-NFKBIA antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NFKBIA Antibody (S32/36) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NFKBIA Antibody (S32/36) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of hela cells using NFKBIA Antibody (S32/36)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of hela cells using NFKBIA Antibody (S32/36)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of NFKBIA-S32/36 in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of NFKBIA-S32/36 in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.)
Related Product Information for anti-NFKBIA antibody
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Product Categories/Family for anti-NFKBIA antibody
References
Szamosi,T., Dig. Dis. Sci. (2008)
Fan,C., J. Biol. Chem. 278 (3), 2072-2080 (2003)
Tojima,Y., Nature 404 (6779), 778-782 (2000)
Gil,J., Oncogene 19 (11), 1369-1378 (2000)
Hay,R.T., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354 (1389), 1601-1609(1999)
Fan,C., J. Biol. Chem. 278 (3), 2072-2080 (2003)
Tojima,Y., Nature 404 (6779), 778-782 (2000)
Gil,J., Oncogene 19 (11), 1369-1378 (2000)
Hay,R.T., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354 (1389), 1601-1609(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35609
NCBI Official Full Name
NF-kappa-B inhibitor alpha
NCBI Official Synonym Full Names
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
NCBI Official Symbol
NFKBIA
NCBI Official Synonym Symbols
IKBA; MAD-3; NFKBI
NCBI Protein Information
NF-kappa-B inhibitor alpha
UniProt Protein Name
NF-kappa-B inhibitor alpha
UniProt Gene Name
NFKBIA
UniProt Synonym Gene Names
IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
UniProt Entry Name
IKBA_HUMAN
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Product Notes
The NFKBIA nfkbia (Catalog #AAA289327) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 12-41. The NFKBIA Antibody (S32/36) reacts with Human (Predicted Reactivity: Mouse, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's NFKBIA can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NFKBIA nfkbia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFKBIA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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