IF (Immunofluorescence)
(AAA322569 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit NFRkappaB Polyclonal Antibody | anti-NFRKB antibody
NFRkappaB Antibody
Gene Names
NFRKB; INO80G
Reactivity
Human, Monkey
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NFRkappaB, Antibody; NFRkappaB Antibody; A530090G11Rik; DKFZp547B2013; DNA binding protein R kappa B; INO80 complex subunit G; INO80G; Nuclear factor related to kappa B binding protein; Nuclear factor related to kappaB binding protein; anti-NFRKB antibody
Host
Rabbit
Reactivity
Human, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
NFRKB antibody detects endogenous levels of total NFRKB.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NFRKB antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human NFRKB, corresponding to a region within the internal amino acids.
Function
Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'.
Similarity
NFRKB seems to be mostly disordered. The wing-helix like domain doesn't bind DNA. Belongs to the NFRKB family.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA322569 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA322569 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis NFRkappaB using COS7 whole cell lysates)
WB (Western Blot)
(Western blot analysis NFRkappaB using COS7 whole cell lysates)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
110 kDa
NCBI Official Full Name
nuclear factor related to kappa-B-binding protein isoform 1
NCBI Official Synonym Full Names
nuclear factor related to kappaB binding protein
NCBI Official Symbol
NFRKB
NCBI Official Synonym Symbols
INO80G
NCBI Protein Information
nuclear factor related to kappa-B-binding protein
UniProt Protein Name
Nuclear factor related to kappa-B-binding protein
UniProt Gene Name
NFRKB
UniProt Synonym Gene Names
INO80G
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Product Notes
The NFRKB nfrkb (Catalog #AAA322569) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFRkappaB Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's NFRkappaB can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the NFRKB nfrkb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFRkappaB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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