WB (Western Blot)
(Anti-NFYA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))
Rabbit NFYA Polyclonal Antibody | anti-NFYA antibody
Anti-NFYA Antibody, Rabbit Polyclonal
Reactivity
HumanPredicted to react with: Mouse, Rat (Species
Predicted to react based on 100% sequence homology)
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
NFYA, Antibody; Anti-NFYA Antibody, Rabbit Polyclonal; NFYA Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-CBF-A Antibody; Anti-CBF-B Antibody; Anti-HAP2 Antibody; Anti-NF-YA Antibody; nuclear transcription factor Y subunit alpha; anti-NFYA antibody
Host
Rabbit
Reactivity
Human
Predicted to react with: Mouse, Rat (Species
Predicted to react based on 100% sequence homology)
Predicted to react with: Mouse, Rat (Species
Predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NFYA
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. 0.2um filtered solution in PBS
Applicable Applications for anti-NFYA antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human NFYA
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NFYA, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
WB (Western Blot)
(Anti-NFYA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-NFYA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(NFYA was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-NFYA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-NFYA rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)
IP (Immunoprecipitation)
(NFYA was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-NFYA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-NFYA rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of NFYA in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human NFYA polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of NFYA in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human NFYA polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Related Product Information for anti-NFYA antibody
Background: The increased expression of the alpha subunit of nuclear transcription factor Y (NFYA) is associated with the malignant phenotype of various tumors. NFYA may promote a malignant phenotype in OS cells, in part, by activating the FASN signaling pathway, which may represent a promising target for the management of OS.
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Product Notes
The NFYA (Catalog #AAA257495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NFYA Antibody, Rabbit Polyclonal reacts with Human Predicted to react with: Mouse, Rat (Species Predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's NFYA can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NFYA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFYA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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