Rabbit anti-Human NGFR Polyclonal Antibody | anti-NGFR antibody

Anti-NGFR Antibody, Rabbit Polyclonal

Cat. Num. AAA254425

• Gene Names: NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NGFR, Antibody; Anti-NGFR Antibody, Rabbit Polyclonal; NGFR/P75/CD271 Antibody, Rabbit PAb, Antigen Affinity Purified; nerve growth factor receptor (TNFR superfamily, member 16); Anti-CD271 Antibody; Anti-Gp80-LNGFR Antibody; Anti-p75(NTR) Antibody; Anti-p75NTR Antibody; Anti-TNFRSF16 Antibody; anti-NGFR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NGFR/P75/CD271
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NGFR antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human NGFR/P75/CD271
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NGFR/P75/CD271, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-CD271 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:75 kDaObserved band size:75 kDa)

IP (Immunoprecipitation)

(CD271 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CD271 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD271 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 75 kDaObserved band size: 75 kDa)

Related Product Information for anti-NGFR antibody

Nerve growth factor receptors (NGFRs) belong to a large growth factor receptor family. NGFR includes two types of receptors: high-affinity nerve growth factor receptor and low-affinity nerve growth factor receptor. The high-affinity nerve growth factor receptor is also referred to as the Trk family whose members are bound by some neurotrophins with high affinity. Nerve growth factor binds with TrkA after being released from target cells, the NGF/TrkA complex is subsequently trafficked back to the cell body. The Low-affinity nerve growth factor receptor also named p75 which binds with all kinds of neurotrophins with low affinity. All four kinds of neurotrophins, including Nerve growth factor, Brain-derived neurotrophic factor, Neurotrophin-3, and Neurotrophin-4 bind to the p75. Studies have proved that NGFR acts as a molecular signal switch that determines cell death or survival by three steps. First, pro-nerve growth factor (prNGF) triggers cell apoptosis by its high-affinity binding to p75NTR, while NGF induces neuronal survival with low-affinity binding. Second, p75NTR mediates cell death by combining with co-receptor Sortilin, whereas it promotes neuronal survival through combination with proNGF. Third, the release of the intracellular domain chopper or cleavage short p75 NTR can independently initiate neuronal apoptosis.

Product Categories/Family for anti-NGFR antibody

Death Receptor Signaling

References

Chen LW, et al. (2008) The proNGF-p75NTR-sortilin signalling complex as new target for the therapeutic treatment of Parkinson's disease. CNS Neurol Disord Drug Targets. 7(6): 512-23.Deponti D, et al. (2009) The low-affinity receptor for neurotrophins p75NTR plays a key role for satellite cell function in muscle repair acting via RhoA. Mol Biol Cell.20(16): 3620-7.Ken-ichiro K, et al. (2004) Necdin-related MAGE proteins differentially interact with the E2F1 transcription factor and the p75 neurotrophin receptor. J Biol Chem. 279 (3): 1703-12.

NCBI and Uniprot Product Information

• NCBI GI #: 4505393
• NCBI GeneID: 4804
• NCBI Accession #: NP_002498.1
• NCBI GenBank Nucleotide #: NM_002507.3
• UniProt Accession #: P08138; B2R961
• Molecular Weight: 45,183 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: NGFR
• NCBI Official Synonym Symbols: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16; p75 ICD; NGF receptor; TNFR superfamily, member 16; low affinity nerve growth factor receptor; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• UniProt Gene Name: NGFR
• UniProt Synonym Gene Names: TNFRSF16; NGF receptor
• UniProt Entry Name: TNR16_HUMAN