IF (Immunofluorescence)
(AAA328059 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA328059) and mouse anti-beta tubulin Ab(# ) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
Rabbit anti-Human NGRN Polyclonal Antibody | anti-NGRN antibody
NGRN Antibody
Gene Names
NGRN; DSC92
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
NGRN, Antibody; NGRN Antibody; DSC92; FI58Gm; Mesenchymal stem cell protein DSC92; Neugrin; Neugrin neurite outgrowth associated; Neurite outgrowth associated protein; Neurite outgrowth-associated protein; Ngrn; NGRN_HUMAN; Spinal cord-derived protein FI58G; anti-NGRN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Supplied in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
~1mg/ml (varies by lot)
Sequence Length
291
Applicable Applications for anti-NGRN antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human NGRN, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328059 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA328059) and mouse anti-beta tubulin Ab(# ) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(AAA328059 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA328059) and mouse anti-beta tubulin Ab(# ) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
IHC (Immunohiostchemistry)
(AAA328059at 1/100 staining human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.)
IHC (Immunohiostchemistry)
(AAA328059at 1/100 staining human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.)
WB (Western Blot)
(Western blot analysis NGRN using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis NGRN using HeLa whole cell lysates)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32 kDa
NCBI Official Full Name
neugrin
NCBI Official Synonym Full Names
neugrin, neurite outgrowth associated
NCBI Official Symbol
NGRN
NCBI Official Synonym Symbols
DSC92
NCBI Protein Information
neugrin
UniProt Protein Name
Neugrin
UniProt Gene Name
NGRN
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Product Notes
The NGRN ngrn (Catalog #AAA328059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NGRN Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NGRN can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NGRN ngrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NGRN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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