Rabbit NHEDC2 Polyclonal Antibody | anti-SLC9B2 antibody

NHEDC2 Antibody (C-term)

Cat. Num. AAA286131

• Gene Names: SLC9B2; NHA2; NHE10; NHEDC2
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NHEDC2, Antibody; NHEDC2 Antibody (C-term); Mitochondrial sodium/hydrogen exchanger 9B2; Mitochondrial Na(+)/H(+) exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; NHE domain-containing protein 2; Sodium/hydrogen exchanger-like domain-containing protein 2; Solute carrier family 9 subfamily B member 2; SLC9B2; NHA2; NHEDC2; anti-SLC9B2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NHEDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-486 amino acids from the C-terminal region of human NHEDC2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 458-486
• Sequence Length: 454

• Applicable Applications for anti-SLC9B2 antibody: ELISA, WB (Western Blot)
• Function: Na(+)/H(+) antiporter that extrudes Na(+) or Li(+) in exchange for external protons across the membrane (PubMed: , PubMed: , PubMed: , PubMed: ). Contributes to the regulation of intracellular pH, sodium homeostasis, and cell volume. Plays an important role for insulin secretion and clathrin-mediated endocytosis in beta-cells (By similarity). Involved in sperm motility and fertility (By similarity). It is controversial whether SLC9B2 plays a role in osteoclast differentiation or not (By similarity).
• Cellular Location: Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Endosome membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multipass membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q5BKR2}. Cell projection, cilium, flagellum membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein. Apical cell membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein. Note=Strong colocalization with LAMP1 and TCIRG1 in osteoclasts. In beta-cells colocalizes with RAB4A and SYP. Localizes to the basolateral membrane of polarized osteoclasts. {ECO:0000250|UniProtKB:Q5BKR2}
• Tissue Location: Widely expressed (PubMed:18508966). High levels detected in the distal tubules of the kidney nephron (PubMed:18508966) Detected in red blood cells (at protein level) (PubMed: 18000046, PubMed:18508966).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(NHEDC2 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the NHEDC2 antibody detected the NHEDC2 protein (arrow).)

Related Product Information for anti-SLC9B2 antibody

Sodium hydrogen antiporters, such as NHEDC2, convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux (Xiang et al., 2007 [PubMed 18000046]).

Product Categories/Family for anti-SLC9B2 antibody

Cell Biology; Metabolism; Signal transduction

References

Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Schushan, M., et al. J. Mol. Biol. 396(5):1181-1196(2010)
Fuster, D.G., et al. J. Am. Soc. Nephrol. 19(8):1547-1556(2008)
Battaglino, R.A., et al. Bone 42(1):180-192(2008)
Xiang, M., et al. Proc. Natl. Acad. Sci. U.S.A. 104(47):18677-18681(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 47271479
• NCBI GeneID: 133308
• NCBI Accession #: NP_849155.2
• NCBI GenBank Nucleotide #: NM_178833.5
• UniProt Accession #: Q86UD5; Q6ZMD8; Q96D95; B5ME52
• Molecular Weight: 57564
• NCBI Official Full Name: mitochondrial sodium/hydrogen exchanger 9B2 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
• NCBI Official Symbol: SLC9B2
• NCBI Official Synonym Symbols: NHA2; NHE10; NHEDC2
• NCBI Protein Information: mitochondrial sodium/hydrogen exchanger 9B2
• UniProt Protein Name: Mitochondrial sodium/hydrogen exchanger 9B2
• UniProt Gene Name: SLC9B2
• UniProt Synonym Gene Names: NHA2; NHEDC2; NHE domain-containing protein 2
• UniProt Entry Name: SL9B2_HUMAN