WB (Western Blot)
(Detection of human NHERF1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NHERF1 antibody AAA212086 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 3 minutes.)
Rabbit anti-Human NHERF1 Polyclonal Antibody | anti-NHERF1 antibody
Rabbit anti-NHERF1 Antibody, Affinity Purified
Gene Names
SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NHERF1, Antibody; Rabbit anti-NHERF1 Antibody, Affinity Purified; Na+/H+ exchange regulatory co-factor; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Sodium-hydrogen exchanger regulatory factor 1; regulatory cofactor of Na(+)/H(+) exchanger; NPHLOP2; NHERF; NHERF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Ezrin-radixin-moesin-binding phosphoprotein 50; ezrin-radixin-moesin binding phosphoprotein-50; EBP50; NHERF-1; SLC9A3 regulator 1; anti-NHERF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-NHERF1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Chicken, Bovine
Immunogen
Between 308 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NHERF1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NHERF1 antibody AAA212086 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human NHERF1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NHERF1 antibody AAA212086 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human NHERF1 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-NHERF1 antibody AAA212086 used for WB at 1 ug/ml and for IP at 3 ug/mg lysate. NHERF1 was also immunoprecipitated by rabbit anti-NHERF1 antibody Detection: Chemiluminescence with exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human NHERF1 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-NHERF1 antibody AAA212086 used for WB at 1 ug/ml and for IP at 3 ug/mg lysate. NHERF1 was also immunoprecipitated by rabbit anti-NHERF1 antibody Detection: Chemiluminescence with exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human NHERF1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NHERF1 (Cat. No. AAA212086 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NHERF1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NHERF1 (Cat. No. AAA212086 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-NHERF1 antibody
NHERF1 (sodium-hydrogen exchanger regulatory factor 1) is a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer [taken from NCBI Entrez Gene (Gene ID: 9368)].
Product Categories/Family for anti-NHERF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358
NCBI Official Full Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
NCBI Official Symbol
SLC9A3R1
NCBI Official Synonym Symbols
EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
NCBI Protein Information
Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-bindi
UniProt Protein Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
UniProt Gene Name
SLC9A3R1
UniProt Synonym Gene Names
NHERF; NHERF1; NHERF-1; EBP50
UniProt Entry Name
NHRF1_HUMAN
Similar Products
Product Notes
The NHERF1 slc9a3r1 (Catalog #AAA212086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NHERF1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NHERF1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NHERF1 slc9a3r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NHERF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.