WB (Western Blot)
(Detection of human NIPBL by western blot. Samples: Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody AAA211627 (lot AAA211627-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human NIPBL Polyclonal Antibody | anti-NIPBL antibody
Rabbit anti-NIPBL Antibody, Affinity Purified
Gene Names
NIPBL; CDLS; IDN3; Scc2; CDLS1; IDN3-B
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NIPBL, Antibody; Rabbit anti-NIPBL Antibody, Affinity Purified; Nipped-B homolog; SCC2 homolog; sister chromatid cohesion 2 homolog; nipped-B-like protein; IDN3; delangin; CDLS1; CDLS; IDN3-B; Scc2; Nipped-B-like protein; NIPBL, cohesin loading factor; anti-NIPBL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NIPBL antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1025 and 1075
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NIPBL by western blot. Samples: Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody AAA211627 (lot AAA211627-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human NIPBL by western blot. Samples: Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody AAA211627 (lot AAA211627-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody AAA211627 (lot AAA211627-4) used for IP at 3 ug per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot AAA211627-3) and rabbit anti-NIPBL antibody For blotting immunoprecipitated NIPBL, AAA211627 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody AAA211627 (lot AAA211627-4) used for IP at 3 ug per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot AAA211627-3) and rabbit anti-NIPBL antibody For blotting immunoprecipitated NIPBL, AAA211627 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-NIPBL antibody
The NIPBL (Nipped B-like) gene was originally identified as a gene mutated in individuals with Cornelia de Lange syndrome (CdLS), a heterogenous developmental disorder associated with multiple anatomical malformations. The NIPBL protein product is also referred to as delangin. It has substantial homology to the D. melanogaster Nipped-B protein which is related to a family of chromosomal adherins that plays a role in sister chromatid adhesion, chromosome condensation, and DNA repair. A study of metaphase spreads from individuals positive and negative for NIPBL mutations revealed an association between NIPBL mutations and precocious sister chromatid separation. This result suggests a function for NIPBL that is similar to its homolog in drosophila.
Product Categories/Family for anti-NIPBL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,367 Da
NCBI Official Full Name
nipped-B-like protein isoform A
NCBI Official Synonym Full Names
NIPBL, cohesin loading factor
NCBI Official Symbol
NIPBL
NCBI Official Synonym Symbols
CDLS; IDN3; Scc2; CDLS1; IDN3-B
NCBI Protein Information
nipped-B-like protein
UniProt Protein Name
Nipped-B-like protein
UniProt Gene Name
NIPBL
UniProt Synonym Gene Names
IDN3
UniProt Entry Name
NIPBL_HUMAN
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Product Notes
The NIPBL nipbl (Catalog #AAA211627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NIPBL Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NIPBL can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NIPBL nipbl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIPBL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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