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FCM/FACS (Flow Cytometry)
(NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit NIX Polyclonal Antibody | anti-BNIP3L antibody
NIX Antibody (Center)
Gene Names
BNIP3L; NIX; BNIP3a
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NIX, Antibody; NIX Antibody (Center); BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; NIP3-like protein X; NIP3L; BNIP3L; BNIP3A; BNIP3H; NIX; anti-BNIP3L antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NIX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the Central region of human NIX.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
52-81
Sequence Length
219
Applicable Applications for anti-BNIP3L antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NIX Antibody (Center) with A549 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NIX Antibody (Center) with A549 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(NIX Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(NIX Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(NIX Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NIX antibody detected the NIX protein (arrow).)
WB (Western Blot)
(NIX Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NIX antibody detected the NIX protein (arrow).)
Related Product Information for anti-BNIP3L antibody
This gene is a member of the BCL2/adenovirus E1B 19
kd-interacting protein (BNIP) family. It interacts with the E1B 19
kDa protein which is responsible for the protection of
virally-induced cell death, as well as E1B 19 kDa-like sequences of
BCL2, also an apoptotic protector. The protein encoded by this
gene is a functional homolog of BNIP3, a proapoptotic protein.
This protein may function simultaneously with BNIP3 and may play a
role in tumor suppression.
kd-interacting protein (BNIP) family. It interacts with the E1B 19
kDa protein which is responsible for the protection of
virally-induced cell death, as well as E1B 19 kDa-like sequences of
BCL2, also an apoptotic protector. The protein encoded by this
gene is a functional homolog of BNIP3, a proapoptotic protein.
This protein may function simultaneously with BNIP3 and may play a
role in tumor suppression.
Product Categories/Family for anti-BNIP3L antibody
References
Novak, I., et al. EMBO Rep. 11(1):45-51(2010)
Bellot, G., et al. Mol. Cell. Biol. 29(10):2570-2581(2009)
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Papandreou, I., et al. Cell Death Differ. 15(10):1572-1581(2008)
Liu, W., et al. Neoplasia 10(8):897-907(2008)
Bellot, G., et al. Mol. Cell. Biol. 29(10):2570-2581(2009)
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Papandreou, I., et al. Cell Death Differ. 15(10):1572-1581(2008)
Liu, W., et al. Neoplasia 10(8):897-907(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23930
NCBI Official Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
NCBI Official Synonym Full Names
BCL2/adenovirus E1B 19kDa interacting protein 3-like
NCBI Official Symbol
BNIP3L
NCBI Official Synonym Symbols
NIX; BNIP3a
NCBI Protein Information
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
UniProt Protein Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
UniProt Gene Name
BNIP3L
UniProt Synonym Gene Names
BNIP3A; BNIP3H; NIX; NIP3L
UniProt Entry Name
BNI3L_HUMAN
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Product Notes
The BNIP3L bnip3l (Catalog #AAA286414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 52-81. The NIX Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's NIX can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the BNIP3L bnip3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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