IHC (Immunohiostchemistry)
(Immunochemical staining of human NLE1 in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human NLE1 Polyclonal Antibody | anti-NLE1 antibody
Anti-NLE1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
NLE1, Antibody; Anti-NLE1 Antibody, Rabbit Polyclonal; NLE1 Antibody, Rabbit PAb, Antigen Affinity Purified; notchless homolog 1 (Drosophila); Anti-Nle Antibody; anti-NLE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NLE1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-NLE1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human NLE1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human NLE1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Immunochemical staining of human NLE1 in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human NLE1 in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-NLE1 rabbit polyclonal antibody at 1:500 dilutionLane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:53 kDaObserved band size:53 kDa)
WB (Western Blot)
(Anti-NLE1 rabbit polyclonal antibody at 1:500 dilutionLane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:53 kDaObserved band size:53 kDa)
Related Product Information for anti-NLE1 antibody
NLE1 (Notchless Homolog 1) is a Protein Coding gene. The NLE1 gene, located on 17q12, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog. NLE1 is a member of the large WD40-repeat protein family and is thought to signal via the canonical NOTCH pathway in vertebrates. NLE1 plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. In the mouse, Notchless homolog 1 (Drosophila) (Nle1) is instrumental for the survival of cells of the inner cell mass upon implantation. Diseases associated with NLE1 include Heart Block, Congenital, and Meningoencephalitis.
References
Romes EM, et al. (2016) The crystal structure of the ubiquitin-like domain of ribosome assembly factor ytm1 and characterization of its interaction with the aaa-atpase midasin. J Biol Chem 291 (2): 882-893.Lossie AC, et al. (2012) Enu mutagenesis reveals that notchless homolog 1 (drosophila) affects cdkn1a and several members of the wnt pathway during murine pre-implantation development. BMC Genet 13 106.Beck-Cormier S, et al. (2014) Notchless is required for axial skeleton formation in mice. PLoS One 9 (5): e98507.
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Product Notes
The NLE1 (Catalog #AAA256527) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NLE1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NLE1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NLE1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NLE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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