Rabbit anti-Human, Mouse NLRC5 / NOD4 Polyclonal Antibody | anti-NLRC5 antibody

Rabbit Polyclonal to Human NLRC5 / NOD4

Cat. Num. AAA51424

• Gene Names: NLRC5; NOD4; NOD27; CLR16.1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Peptide Column Affinity Chromatography
• Synonyms: NLRC5 / NOD4, Antibody; Rabbit Polyclonal to Human NLRC5 / NOD4; Anti-NLRC5 / NOD4 Antibody (N-Terminus) IHC-plus; NLRC5; CLR16.1; NOD-like receptor C5; NOD27; NOD4; Protein NLRC5; Caterpiller protein 16.1; Human NLRC5; anti-NLRC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NOD4 antibody is predicted to not cross-react with other members of the NOD family of proteins.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1866

• Applicable Applications for anti-NLRC5 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen Species: NLRC5 / NOD4 antibody was raised against Human
• Immunogen Type: Synthetic peptide
• Immunogen: NLRC5 / NOD4 antibody was raised against an 18 AA synthetic peptide near the N-Terminus of human NOD4. The immunogen is located within aa 20-70 of NOD4.
• Usage: NOD4 antibody can be used for detection of NOD4 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 205 kDa
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of NOD4 in EL4 cell lysate with NOD4 antibody at 1 ug/ml.)

ICC (Immunocytochemistry)

(Immunocytochemistry of NOD4 in HeLa cells with NOD4 antibody at 10 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescence of NOD4 in HeLa cells with NOD4 antibody at 20 ug/ml.)

IHC (Immunohistochemistry)

(Anti-NLRC5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 116268109
• NCBI GeneID: 84166
• NCBI Accession #: NP_115582.3
• NCBI GenBank Nucleotide #: NM_032206.3
• UniProt Accession #: Q86WI3; Q6P4A6; Q86VM7; Q8NF42; Q8TEE2; Q8TEJ1; Q969L7; B5MEF1; C9JMD8
• NCBI Official Full Name: protein NLRC5
• NCBI Official Synonym Full Names: NLR family, CARD domain containing 5
• NCBI Official Symbol: NLRC5
• NCBI Official Synonym Symbols: NOD4; NOD27; CLR16.1
• NCBI Protein Information: protein NLRC5; NOD-like receptor C5; caterpiller protein 16.1; nucleotide-binding oligomerization domains 27; nucleotide-binding oligomerization domain protein 4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 5
• UniProt Protein Name: Protein NLRC5
• UniProt Gene Name: NLRC5
• UniProt Synonym Gene Names: NOD27; NOD4; CLR16.1
• UniProt Entry Name: NLRC5_HUMAN