Rabbit anti-Human NLRP3 Polyclonal Antibody | anti-NLRP3 antibody

NLRP3 Antibody

Cat. Num. AAA232603

• Gene Names: NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, ELISA
• Purity: >95%, Protein G purified
• Synonyms: NLRP3, Antibody; NLRP3 Antibody; NACHT; LRR and PYD domains-containing protein 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; Cold-induced autoinflammatory syndrome; NLRP3; C1orf7; CIAS1; NALP3; PYPAF1; anti-NLRP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 1036

• Applicable Applications for anti-NLRP3 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
• Immunogen: Recombinant human NACHT, LRR and PYD domains-containing protein 3 protein (30-102AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-34411 / sc-34410 / sc-34408 / sc-66846
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IF (Immunofluorescence)

(Immunofluorescent analysis of HepG2 cells using AAA232603 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human bladder cancer using AAA232603 at dilution of 1:100)

Related Product Information for anti-NLRP3 antibody

As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed: 22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, beta-amyloid fibers, environmental or industrial particles and nanoparticles, etc. However, it is unclear what constitutes the direct NLRP3 activator. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (By similarity). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 119395764
• NCBI GeneID: 114548
• NCBI Accession #: NP_001073289.1
• NCBI GenBank Nucleotide #: NM_001079821.2
• UniProt Accession #: Q96P20; O75434; Q17RS2; Q59H68; Q5JQS8; Q5JQS9; A0A024R5Q0; B2RC97; B7ZKS9; B7ZKT2; B7ZKT3
• Molecular Weight: 115,968 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3 isoform a
• NCBI Official Synonym Full Names: NLR family pyrin domain containing 3
• NCBI Official Symbol: NLRP3
• NCBI Official Synonym Symbols: AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• UniProt Gene Name: NLRP3
• UniProt Synonym Gene Names: C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1
• UniProt Entry Name: NLRP3_HUMAN