Application Data
(Left Image: Immunostaining of 14 DIV rat cortical neurons showing NR2B (green, 1:400) and PSD95(red). Photo courtesy of Gang Liu.Right Image: Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.)
Rabbit NMDA NR2B Subunit, N-terminus Polyclonal Antibody | anti-GRIN2B antibody
Anti-NMDA Receptor, NR2B Subunit N-terminus
Gene Names
Grin2b; GluN2B
Reactivity
Mouse, RatExpected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)
Synonyms
NMDA NR2B Subunit, N-terminus, Antibody; Anti-NMDA Receptor, NR2B Subunit N-terminus; anti-GRIN2B antibody
Host
Rabbit
Reactivity
Mouse, Rat
Expected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate
Expected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate
Clonality
Polyclonal
Specificity
Specific for the ~180k NR2B subunit of the NMDA receptor in Western Blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the N-terminal peptide that was used to generate the antibody.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)
Form/Format
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
Sequence Length
1482
Applicable Applications for anti-GRIN2B antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).
Preparation and Storage
Store at -20 degree C; stable for at least 1 year
Application Data
(Left Image: Immunostaining of 14 DIV rat cortical neurons showing NR2B (green, 1:400) and PSD95(red). Photo courtesy of Gang Liu.Right Image: Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.)
Application Data
(Left Image: Immunostaining of 14 DIV rat cortical neurons showing NR2B (green, 1:400) and PSD95(red). Photo courtesy of Gang Liu.Right Image: Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.)
Related Product Information for anti-GRIN2B antibody
Affinity purified rabbit polyclonal antibody
References
• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Clayton DA, Grosshans DR, Browning MD (2002a) Aging and surface expression of hippocampal NMDA receptors. J Biol Chem 277:14367-14369.
• Clayton DA, Mesches MH, Alvarez E, Bickford PC, Browning MD (2002b) A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat. J Neurosci 22:3628-3637.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Clayton DA, Grosshans DR, Browning MD (2002a) Aging and surface expression of hippocampal NMDA receptors. J Biol Chem 277:14367-14369.
• Clayton DA, Mesches MH, Alvarez E, Bickford PC, Browning MD (2002b) A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat. J Neurosci 22:3628-3637.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
180 kDa
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2B
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol
Grin2b
NCBI Official Synonym Symbols
GluN2B
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2B
UniProt Gene Name
Grin2b
UniProt Synonym Gene Names
GluN2B; NMDAR2B; NR2B
UniProt Entry Name
NMDE2_RAT
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Product Notes
The GRIN2B grin2b (Catalog #AAA72798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NMDA Receptor, NR2B Subunit N-terminus reacts with Mouse, Rat Expected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate and may cross-react with other species as described in the data sheet. AAA Biotech's NMDA NR2B Subunit, N-terminus can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GRIN2B grin2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMDA NR2B Subunit, N-terminus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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